| 山羊骨髓间充质干细胞生物学性能鉴定及诱导分化为心肌细胞 |
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| 引用本文： | 庞全海,张慧茹,华进联,马勇江,窦忠英. 山羊骨髓间充质干细胞生物学性能鉴定及诱导分化为心肌细胞[J]. 中国农学通报, 2005, 21(5): 1-1 |
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| 作者姓名： | 庞全海 张慧茹 华进联 马勇江 窦忠英 |
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| 作者单位： | （1西北农林科技大学国家干细胞工程技术研究中心陕西分中心，杨凌 712100；2山西农业大学动物科技学院，太谷 030801；3华南农业大学兽医学院，广州 510642） |
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| 摘 要： | 研究了关中奶山羊骨髓间充质干细胞的分离、培养和生物学特性，并在体外诱导分化为心肌细胞表型。结果表明，山羊MSCs具有很好增殖能力；能够耐受反复冷冻和解冻；表达细胞表面标志CD44、CD105、CD166，弱表达CD34、CD106，而不表达CD14、CD45。该类细胞用5-氮胞苷诱导分化后，能够表达心肌α-actin，并呈PAS染色阳性。
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| 关 键 词： | 山羊 骨髓间充质干细胞 分化潜能 鉴定 心肌细胞 |
Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro |
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| Pang Quanhai,Zhang Huiru,Hua Jinlian,Ma Yongjiang,Dou Zhongying. Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro[J]. Chinese Agricultural Science Bulletin, 2005, 21(5): 1-1 |
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| Authors: | Pang Quanhai Zhang Huiru Hua Jinlian Ma Yongjiang Dou Zhongying |
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| Affiliation: | (1Shaanxi Branch of National Stem Cells Engineering and Technology Research Center, Yangling Shaanxi 712100;2Animal Science and Technology College of Shanxi Agricultural University, Taigu Shanxi 030801;3Veterinary College of South China Agricultural Uni |
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| Abstract: | The Guanzhong dairy goats were used in our experiment to study the characteristics of their bone marrow mesenchymal stem cells (BM-MSCs), such as isolation, culture, and identification. The results indicate that there are excellent potentials for the goat mesenchymal-like stem cells derived from goat bone marrow to proliferate and to resist freezing and thawing repeatedly. The goat mesenchymal-like stem cells can express cellular surface markers CD34, CD44, CD105, CD106, CD 166, and TGF-α, but not CD14 and CD45. And after treated by 5-azacytidine in vitro, they express cellular surface marker of cardiac α-actin and Schiff’s stain positive. |
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| Keywords: | Goatszz MSCszz Differentiation Potentialzz Identificationzz Cardiomyocytezz |
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