玉米植株再生体系建立及其主要影响因素的优化

研究选取11个东北地区主栽玉米品种的自交系,以幼胚为外植体诱导愈伤组织,应用正交实验设计方法探讨了2,4-D、BA、L-脯氨酸、水解酪蛋白4个因素及不同浓度组合对II型愈伤组织诱导的影响。结果表明,MS培养基中添加4mg·L-12,4-D、0.5mg·L-1BA、750mg·L-1L-脯氨酸和250mg·L-1水解酪蛋白时,II型愈伤组织诱导率最高,为49.66%。2,4-D是诱导II型愈伤组织的关键因素。优化了不定芽分化培养基和生根培养基,探讨了3个基因型愈伤组织的不定芽分化率及平均外植体出芽数,甜1品种都为最高,分别为93.14%和1.35%。初步确定了蛭石附加1/8MS营养液为再生植株移栽的最佳条件。

Stablishment of maize(Zea mays L.)regeneration system and optimization of its main influencing factors

Eleven main maize cultivars in northeast district of China were sele cted and the immature embryos was used as explants in order to induce callus. Th e effect of four factors, 2, 4-D, BA , L-Pro and CH and their different combinat ion of concentration on type II callus induction were analyzed. The results show ed that optimal medium for type II callus induction was MS adding 4 mg·L-1 2, 4-D, 5 mg·L-1 BA, 750 mg·L-1 L-Pro and 250 mg·L-1 CH, and the highest inductio n rate is 49.66%. 2, 4-D is the key factor of type II callus induction. Differen tiation medium for buds and root were also optimized respectively. Differentiati on rate of callus and the average differentiation buds number of each callus of three genotypes maize were discussed. Genotype Tian1 has the highest, differenti ation rate is 93.14 % and the most buds of explant is 1.35%. The optimum transpl antation medium for regeneration seedling is vermiculite soaked with 1/8 MS.