| 芜菁黄烷酮3-羟化酶基因的克隆、序列分析及表达 |
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| 引用本文： | 许志茹,崔国新,李春雷,孙燕,李玉花. 芜菁黄烷酮3-羟化酶基因的克隆、序列分析及表达[J]. 分子植物育种, 2008, 6(4) |
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| 作者姓名： | 许志茹 崔国新 李春雷 孙燕 李玉花 |
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| 作者单位： | 东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨,150040;东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨,150040;东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨,150040;东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨,150040;东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金 , 国家自然科学基金 |
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| 摘 要： | 花青素是一类重要的植物次生代谢产物,黄烷酮3-羟化酶（F3H）是花青素生物合成早期阶段的重要催化酶。利用UV-A处理津田芜菁（Tsuda turnip）和赤丸芜菁（Yurugi Akamaru turnip）块根24h后提取总RNA,通过RT-PCR方法克隆了BrF3H基因。津田芜菁和赤丸芜菁的F3H基因完全相同。BrF3H的开放读码框为1077bp,编码358个氨基酸。氨基酸序列分析显示,BrF3H与甘蓝型油菜（Brassica napus）F3H-1的同源性为99%。Northern杂交结果显示,UV-A可以诱导BrF3H表达,基因的表达量与处理时间呈相关。
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| 关 键 词： | 芜菁 黄烷酮3-羟化酶基因 克隆与表达 序列分析 |
Cloning, Sequence Analysis and Expression of Flavanone 3-hydroxylase Gene in Turnip |
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| Xu Zhiru,Cui Guoxin,Li Chunlei,Sun Yan,Li Yuhua. Cloning, Sequence Analysis and Expression of Flavanone 3-hydroxylase Gene in Turnip[J]. Molecular Plant Breeding, 2008, 6(4) |
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| Authors: | Xu Zhiru Cui Guoxin Li Chunlei Sun Yan Li Yuhua |
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| Affiliation: | Xu Zhiru Cui Guoxin Li Chunlei Sun Yan Li Yuhua College of Life Sciences,Northeast Forestry University,Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education,Harbin,150040 |
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| Abstract: | Anthocyanins are a class of important secondary metabolites in plants, and flavanone 3-hydroxylase (F3H) is a committed catalyze enzyme in early stage of anthocyanins biosynthesis. The roots of Tsuda turnip and Yurugi Akamaru turnip were irradiated with UV-A light for 24 h. Total RNA was isolated and BrF3H genes were cloned by RT-PCR method. The F3H gene of Tsuda turnip was same to that of Yurugi Akamaru turnip. The open reading frame (ORF) of BrF3H gene contained 1 077 bp and encoded a protein of 358 amino... |
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