| AFLP,SSR在黄瓜黑星病抗感材料上的多态性比较 |
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| 引用本文： | 孙小红,杜胜利,张桂华,王葆利,郭蔼光,裴国亮.AFLP,SSR在黄瓜黑星病抗感材料上的多态性比较[J].华北农学报,2006,21(3):105-107. |
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| 作者姓名： | 孙小红 杜胜利 张桂华 王葆利 郭蔼光 裴国亮 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西,杨凌,712100
2. 天津科润黄瓜研究所,天津,300192 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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天津市农业科学院院长基金 |
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| 摘 要： | 用AFLP和SSR 2种分子标记技术对黄瓜抗感黑星病材料Q6和Q12,及其F2极性集团和F2群体进行了分析,比较了它们的多态性。结果表明,AFLP和SSR 2种分子标记的多态性比率分别为36.5%,9.6%;阳性比率分别为22%,0。在F2群体中找到了1个AFLP标记E20/M64,与目的基因的遗传距离是4.83 cM;1个SSR标记CSWCT02B,与目的基因的遗传距离是28.7 cM。AFLP的多态性比率要比SSR的多态性比率高。分析探讨了2种分子标记技术的优缺点及其在目的基因连锁标记筛选、基因定位等研究中的应用。
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| 关 键 词： | 黄瓜 黑星病 分子标记 AFLP SSR |
| 文章编号： | 1000-7091（2006）03-0105-03 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005-09-26 |
Polymorphism Comparison of AFLP and SSR Between Scab Resistant Cucumber Materials |
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| SUN Xiao-hong,DU Sheng-li,ZHANG Gui-hua,WANG Bao-li,GUO Ai-guang,PEI Guo-liang.Polymorphism Comparison of AFLP and SSR Between Scab Resistant Cucumber Materials[J].Acta Agriculturae Boreali-Sinica,2006,21(3):105-107. |
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| Authors: | SUN Xiao-hong DU Sheng-li ZHANG Gui-hua WANG Bao-li GUO Ai-guang PEI Guo-liang |
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| Institution: | 1. College of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2.Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China |
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| Abstract: | AFLP and SSR analysis were performed among scab resistant parents,F_(2) plants and their bulked extremes.Their rates of polymorphism and positive marker linked to target gene was compared.The results indicated that the rates of polymorphism of AFLP,SSR were 36.5% and 22% respectively;the positive rates were 9.6% and 0.AFLP primer pair E20M64 was found closely linked to target gene,genetic distance was 4.83 cM.SSR primer CSWCT02B was linked to target gene with genetic distance of 28.7 cM.Polymorphism and positive marker rates of AFLP was higher than that of SSR.The advantages and disadvantages,and application of two kinds of molecular marker system in selection of linkage markers to target gene and gene tagging were also discussed. |
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| Keywords: | Cucumber Scab Molecular marker AFLP SSR |
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