| 液体培养基对有斑百合花粉管生长的影响 |
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| 引用本文： | 李晶,刘冬云,张灿. 液体培养基对有斑百合花粉管生长的影响[J]. 河北农业大学学报, 2011, 34(6): 50-54 |
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| 作者姓名： | 李晶 刘冬云 张灿 |
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| 作者单位： | 1. 河北农业大学园林与旅游学院,河北保定,071000
2. 保定职业技术学院,河北保定,071000 |
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| 基金项目： | 河北省教育厅项目(2010253) |
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| 摘 要： | 以有斑百合花粉为试材,通过离体培养,利用单因子试验研究蔗糖、硼酸、以及钙、镁、钾离子对花粉萌发和花粉管生长的影响.并在此基础上进行多因子试验比较了蔗糖、H3 BO3、CaCl2和Ca(NO3)2·4H2O对有斑百合花粉萌发的影响.结果表明:蔗糖、硼和钙对有斑百合花粉萌发影响较大,而钾和镁的影响较小;适宜的花粉培养液为蔗...
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| 关 键 词： | 有斑百合 花粉 液体培养基 生命力 |
Effects of culture solution on pollen tube growth of Lilium concolor var. buschianum Baker |
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| LI Jing , LIU Dong-yun , ZHANG Can. Effects of culture solution on pollen tube growth of Lilium concolor var. buschianum Baker[J]. Journal of Agricultural University of Hebei, 2011, 34(6): 50-54 |
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| Authors: | LI Jing LIU Dong-yun ZHANG Can |
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| Affiliation: | 1.College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071000,China; 2.Baoding Vocational and Technical College,Baoding 071000,China) |
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| Abstract: | The pollen of Lilium concolor var.buschianum Baker was employed as experimental material to study culture solution for the determination of lily pollen viability.Comparisons were made among the effects of different concentration of sucrose,boric acid,calcium,magnesium and potassium on the germination and tube growth of Lilium concolor var.buschianum Baker in vitro.And multi-factor design was used on such basis to compare the effects of sucrose,boric,CaCl2 and Ca(NO3)2·4H2O on the germination of lily pollen.Results showed that sucrose,boric acid and calcium have great effects on the germination of lily pollen,but potassium and magnesium have few effects on the germination of lily pollen.And the optimum culture solution was sucrose 100 g/L+H3BO320 mg/L+CaCl220 mg/L.So reference basis for pollen culture in vitro and crossbreeding of Lilium concolor var.buschianum Baker was offered. |
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| Keywords: | Lilium concolor var.buschianum Baker pollen culture solution vitality |
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