小麦不同生育时期木聚糖酶活性及木聚糖酶抑制蛋白活性的变化

为给小麦抑制蛋白的分离纯化以及表达研究提供信息,以小麦品种兰考矮早八和豫麦49为材料,检测和分析了小麦在不同生育时期叶片和籽粒木聚糖酶活性及木聚糖酶抑制蛋白活性的动态变化.结果表明,木聚糖酶活性及木聚糖酶抑制蛋白活性在两个品种间没有差异,不同生育时期小麦叶片及籽粒木聚糖酶活性没有显著变化,可能以基础水平存在.叶片中的木聚糖酶抑制蛋白活性自苗期开始,经拔节期到抽穗期,总体呈现增加趋势,但进入灌浆～成熟期则显著下降,而籽粒中的木聚糖酶抑制蛋白活性则随籽粒的成熟持续升高.

Changes of Xylanase Activity and Xylanase Inhibitor Activity in Wheat at Different Growth Stages

To find out the existence of xylanase and xylanase inhibitor protein in domestic wheat cultivars, two varieties, Lankaoaizao 8 and Yumai 49, were chosen as materials to investigate the dynamic changes of xylanase and xylanase inhibitor activity in both leaves and kernels of the field planted wheat at different growth stages. The results showed that there were no difference in xylanase and xylanase inhibitor activity between the two wheat varieties, and that no significant changes of xylanase activity whether in leaves or kernels were observed during different growth stages, indicating that xylanase might exist in wheat leaves and kernels as a basal level. The xylanase inhibitor activity in leaves increased from seedling stage, through jointing stage to heading stage, but significantly decreased at grain filling maturing stage, while the xylanase inhibitor activity in kernels increased continuously with the maturation of kernels.