| 二倍体马铃薯花药培养再生体系的优化 |
| |
| 引用本文： | 李文霞,宁海龙,张永根,白雅梅,石瑛,魏峭嵘,张丽莉,吕文河. 二倍体马铃薯花药培养再生体系的优化[J]. 中国蔬菜, 2012, 1(8): 68-74 |
| |
| 作者姓名： | 李文霞 宁海龙 张永根 白雅梅 石瑛 魏峭嵘 张丽莉 吕文河 |
| |
| 作者单位： | 1.东北农业大学农学院,黑龙江哈尔滨 150030,2东北农业大学动物科技学院,黑龙江哈尔滨 150030,3东北农业大学资环学院,黑龙江哈尔滨 150030 |
| |
| 基金项目： | 黑龙江省博士后资助项目(LBH-Z07243);东北农业大学科学研究基金项目;黑龙江省教育厅科学技术研究项目(11521036) |
| |
| 摘 要： | 选取与花药培养相关的因素及水平进行探讨,以优化二倍体马铃薯花药培养的再生体系。结果表明：二倍体马铃薯花药培养的最适基因型为BD40-2、BD54-8和BD10-7|对于促进花药诱导,低温、热激、离心、甘露醇4种预处理之间存在极显著差异,确定最佳的预处理为低温6 d、热激4 d、离心4 000 r?min-1、甘露醇处理2 d|2,4-D为1.0 mg?L-1、6-BA为0.1 mg?L-1的激素配比的花药诱导率最高|碳源选用麦芽糖,浓度为3%|培养基中添加1 mmol?L-1 STS诱导的愈伤花药数显著高于培养基中添加50 mg?L-1 AgNO3。
|
| 关 键 词： | 二倍体 马铃薯 花药培养 褐化 诱导 |
Optimization of Regeneration System for Diploid Potato Anther Culture |
| |
| LI Wen-xia,NING Hai-long,ZHANG Yong-gen,BAI Ya-mei,SHI Ying,WEI Qiao-rong,ZHANG Li-li,LV Wen-he. Optimization of Regeneration System for Diploid Potato Anther Culture[J]. China Vegetables, 2012, 1(8): 68-74 |
| |
| Authors: | LI Wen-xia NING Hai-long ZHANG Yong-gen BAI Ya-mei SHI Ying WEI Qiao-rong ZHANG Li-li LV Wen-he |
| |
| Affiliation: | 1.College of Agronomy,Northeast Agricultural University,Harbin 150030,Heilongjiang,China|2College of Animal Sciences,Northeast Agricultural University,Harbin 150030,Heilongjiang,China|3College of Resources and Environmente Sciences,Northeast Agricultural University,Harbin 150030,Heilongjiang,China |
| |
| Abstract: | In this experiment,factors and levels related to anther culture were selected to discuss the optimization of regeneration system for diploid potato(Solanum tuberosum L.)anther culture.The results indicated that the optimal genotypes for diploid anther culture were BD40-2,BD54-8 and BD10-7.Four pretreatments including low temperature,heat shock,decentralization and mannitol had significant differences on callus inducing.And the optimal combination were low temperature for 6 days,heat shock for 4 days,centrifugation for 4 000 r.min-1and mannitol for 2 days.The horhome combination was 2,4-D 1.0 mg.L-1 + 6-BA 0.1 mg.L-1,which has the highest rate of anther induction.Conformable carbon source was barley sugar at the level of 3%.Adding 1 mmol.L-1 STS in medium had advantages in callus inducing over 50 mg.L-1AgNO3. |
| |
| Keywords: | Diploid Potato Anther culture Browning Inducing |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
|
点击此处可从《中国蔬菜》下载全文 |
|
