| 花叶络石愈伤组织培养初探 |
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| 引用本文： | 杨锋利,杜保国,罗国荣,何晓芸,罗雪梅. 花叶络石愈伤组织培养初探[J]. 北方园艺, 2010, 0(23): 143-145 |
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| 作者姓名： | 杨锋利 杜保国 罗国荣 何晓芸 罗雪梅 |
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| 作者单位： | 绵阳师范学院,城乡建设与规划学院,四川,绵阳,621000;绵阳师范学院,城乡建设与规划学院,四川,绵阳,621000;绵阳师范学院,城乡建设与规划学院,四川,绵阳,621000;绵阳师范学院,城乡建设与规划学院,四川,绵阳,621000;绵阳师范学院,城乡建设与规划学院,四川,绵阳,621000 |
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| 摘 要： | 以多年生花叶络石幼嫩茎段和叶片为外植体,探讨了不同消毒方式、不同外植体类型以及不同激素浓度配比对愈伤组织诱导的影响。结果表明:75%的酒精处理30 s+0.1%HgCl2处理10 min,最适合幼嫩茎段的消毒;75%的酒精处理30 s+0.1%HgCl2处理6 min,最适合幼嫩叶片的消毒;其较理想的愈伤组织增殖培养基为MS+6-BA 1.0 mg/L+2,4-D 1.0 mg/L。
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| 关 键 词： | 花叶络石 愈伤组织 诱导 增殖 |
Callus Induction and Proliferation of Trachelospermum jasminoides Flame |
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| YANG Feng-li,DU Bao-guo,LUO Guo-rong,HE Xiao-yun,LUO Xue-mei. Callus Induction and Proliferation of Trachelospermum jasminoides Flame[J]. Northern Horticulture, 2010, 0(23): 143-145 |
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| Authors: | YANG Feng-li DU Bao-guo LUO Guo-rong HE Xiao-yun LUO Xue-mei |
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| Affiliation: | YANG Feng-li,DU Bao-guo,LUO Guo-rong,HE Xiao-yun,LUO Xue-mei(College of Contraction and Planning of Urban and Rural,Mianyang Normal University,Mianyang,Sichuan 621000) |
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| Abstract: | We used the young stems and leaves of Trachelospermum jasminoides Flame as experiment material to compare effect of different disfection methods,different hormones on callus inducing and proliferating.The results showed that the disinfection method of 75% of the alcohol to deal with 30 s and 0.1% mercuric chloride to deal with 10 min was the most suitable for young stems.The disinfection method of 75% of the alcohol to deal with 30 s and 0.1% mercuric chloride to deal with 6 min was the most suitable for yo... |
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| Keywords: | Trachelospermum jasminoides Flame callus induction proliferation |
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