| 香石竹品种的RAPD 标记 |
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| 引用本文： | 苏友波,林春,毛静,莫锡君,杨明.香石竹品种的RAPD 标记[J].园艺学报,2004,31(1):109-112. |
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| 作者姓名： | 苏友波 林春 毛静 莫锡君 杨明 |
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| 作者单位： | ( 云南农业大学资源与环境学院, 昆明650201 ; 云南农业大学农学与生物技术学院, 昆明650201 ; 3 云南省农业科学院园艺所, 昆明650201) |
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| 基金项目： | 云南省科技攻关资助项目 ( 95A2 -7) |
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| 摘 要： | 用随机扩增多态DNA (RAPD) 技术, 选用4 个随机引物, 对87 个大花型香石竹品种总DNA进行随机扩增。4 个引物均得到了稳定的RAPD 图谱。扩增出的片段分子量在500～4300 bp 之间, 每个随机引物扩增出的条带数在8～12 条之间, 共扩增出2113 个条带, 平均每个引物扩增的DNA 条带数为915 条。根据DNA 谱带计算品种间遗传距离, 对87 个品种进行了聚类分析, 分为10 个组群, 组群间差异较大, 组群内差异较小。
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| 关 键 词： | 香石竹 RAPD 遗传多样性 |
| 文章编号： | 0513-353X(2004)01-0109-03 |
| 收稿时间： | 2003-5-9 |
| 修稿时间： | 2003-10-30 |
RAPD Identification of Different Carnation Cultivars |
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| Su Youbo ,Lin Chun ,Mao Jing ,Mo XiJun ,and Yang Ming.RAPD Identification of Different Carnation Cultivars[J].Acta Horticulturae Sinica,2004,31(1):109-112. |
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| Authors: | Su Youbo Lin Chun Mao Jing Mo XiJun and Yang Ming |
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| Institution: | 1. Department of Pathology, Beijing University of Chinese Medicine, Beijing 100029, China;
2. The First Hospital of Zibo City, Shandong 255200, China |
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| Abstract: | The technique of random amplified polymorphic DNA(RAPD)was used to amplify the genomic DNA fragments of 87 carnation cultivars by 4 selected arbitrary 10-mer primers,2113 bands were obtained, and the size range was 500-4300 bp.The average number of DNA bands amplified by each primer was 9.5 cluster analysis showed that the extensive genetic variation existed among 87 cultivars of carnation.The 87 cultivars were divided into 10 groups at DNA level.This result has significance for the genetic breeding and identification of cultivars of carnation. |
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| Keywords: | Carnation(Dianthus caryophyllus L ) RAPD Genetic diversity |
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