首页 | 本学科首页   官方微博 | 高级检索  
     检索      


The Advantages of Combining Low‐Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen
Authors:D Bencharif  L Amirat  O Pascal  M Anton  E Schmitt  S Desherces  G Delhomme  M‐L Langlois  P Barrière  M Larrat  D Tainturier
Institution:1. Laboratory of Biotechnology and Pathology of Reproduction, National Veterinary School of Nantes;2. Groupe Physico‐chimie des Emulsions, Laboratoire d’Etude des Interactions des Molécules Alimentaires, Institut National de la Recherche Agronomique (INRA), Nantes;3. IMV Technologies, Aigle;4. Department of Reproductive Medicine and Biology CHU HME, Nantes, France
Abstract:Twenty sperm samples from five dogs were frozen in liquid nitrogen at ?196°C in 16 different media, two control media containing 20% egg yolk and 6% low‐density lipoproteins (LDL); 10 test media containing 6% LDL (the active cryoprotective ingredient of chicken egg yolk) combined with 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 mmol of glutamine respectively at 4%, 5%, 7%, and 8% LDL. Following thawing, sperm mobility was assessed using an image analyser, HAMILTON THORN CERROS 12. The percentage of mobile spermatozoa was 62.05% in the 6% LDL + 20 mmol glutamine medium compared with 48.90% in the egg yolk‐based medium (p < 0.05) or 57.55% for the 6% LDL medium (p < 0.05). Furthermore, in most cases, the motility parameters (average path velocity, curvilinear velocity, straight line velocity) in the 6% LDL + 20 mmol glutamine medium, were superior, to a statistically significant extent, to those in the control media. Finally, the 6% LDL + 20 mmol glutamine combination provides spermatozoa with better protection during freezing than egg yolk or the 6% LDL medium alone in terms of acrosome integrity (fluorescein isothiocyanate–Pisum sativum agglutinin test: p < 0.05), the flagellar plasma membrane (hypo‐osmotic test: p < 0.05 for 6% LDL), the DNA (acridine orange test; no significant difference) and the integrity of the acrosome (Spermac® test: no significant difference).
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号