Oxyntomodulin reduces expression of glucagon‐like peptide 1 receptor in the brainstem of chickens |
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| Authors: | A. G. Moghaddam M. M. Yaghoobi H. Jonaidi M. T. Z. Mahani H. Sepehri |
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| Affiliation: | 1. School of Biology, University College of Sciences, University of Tehran, Tehran Iran,;2. School of Sciences, Department of biology, Shahid Bahonar University of Kerman, Kerman, Iran,;3. Department of Biotechnology, Research Institute of Environmental Sciences, International Centre for Science, High Technology and Environmental Sciences, Kerman, Iran, and;4. Group of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman (SBUK), Kerman, Iran;5. Department of Biotechnology, Research Institute of Environmental Sciences, International Centre for Science, High Technology and Environmental Sciences, Kerman, Iran, and |
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| Abstract: | Oxyntomodulin (OXM) is a peptide released from the gut and attenuates food intake by acting on hypothalamus. However, its role at the molecular level is not well studied. In the first section of this study, we analysed the effect of OXM on food intake behaviour after injecting into the lateral ventricle of chickens. The outcome showed that food intake decreased significantly after administering 4 nmol of OXM. In the second part, the expression of glucagon‐like peptide 1 receptor (GLP‐1R) in the brainstem was analysed by real‐time RT‐PCR. The results showed that expression of GLP‐1R was reduced to 27% and 16% at 30 and 90 mins after injection of OXM respectively. In saline‐injected chickens, no reduction in GLP‐1R was seen. It can be concluded that OXM has a down regulatory effect on the responding receptor, GLP‐1R and OXM in chicks has the same reductive effect on food intake as in the mammals. |
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| Keywords: | oxyntomodulin food intake glucagon‐like peptide 1 receptor expression brainstem chicken |
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