| 抗沙门氏菌O_4、O_(12)抗原特异单克隆抗体的产生及其特性鉴定 |
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| 引用本文： | 张如宽,甘军纪,刘秀梵. 抗沙门氏菌O_4、O_(12)抗原特异单克隆抗体的产生及其特性鉴定[J]. 扬州大学学报(农业与生命科学版), 1988, 0(1) |
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| 作者姓名： | 张如宽 甘军纪 刘秀梵 |
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| 作者单位： | Department of Animal Husbandry and Veterinary Medicine，Jiangsu Agricultural College |
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| 摘 要： | 用鼠伤寒沙门氏菌的热灭活全菌和热酚—水抽提脂多糖抗原免疫BALB/c小鼠,取脾脏淋巴细胞与SP_2/O细胞融合,获得稳定分泌抗沙门氏菌O_4和O_(12)抗原的特异单克隆抗体的杂交瘤细胞系7个,分别命名为AG24-1、AG90-5、BG1-8、BG17-12、BG66-10、BG78-15、EG19-11。小鼠腹水中的单抗直接凝集滴度为10~2-10~4,间接免疫荧光滴度为10~3-10~6。与沙门氏菌和肠杆菌科中其他细菌的反应性试验表明,BG78-15是O_(12)因子特异单抗,其他6种为对O_4因子的特异单抗。
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| 关 键 词： | 沙门氏菌 O抗原 单克隆抗体 |
Monoclonal Antibodies with Specificity to O_4 and O_(12) Antigens of Salmonella Species |
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| Zhang Rukuan Gan Junji Liu Xiufan. Monoclonal Antibodies with Specificity to O_4 and O_(12) Antigens of Salmonella Species[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 1988, 0(1) |
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| Authors: | Zhang Rukuan Gan Junji Liu Xiufan |
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| Abstract: | 7 hybridoma cell lines, AG24-1, AG90-5, BG1-8,BG17-12, BG66-10, BG78-15, and EG19-11, secreting monoclonal anti odies with specificity to O_4 and O_(12) antigens of salmenella species were derived from 2 separate fusions between SP_2/O myeloma cells and spleen cells from mice immunized with heat-killed bacteria cells and/or phenol-extracted lipopolysaccharide of salmonella typhimurium. The monoclonai antibodies from mouse aseitic fluid had titers Of up to 10~4 in the indirect agglutination test and titers ranging from 10~3 to 10~6 in the indirect immunofluorescenee test. Binding assays showed that BG78-15 was capable of reacting with all salmonella species bearing O_(12) antigen, and that all the othermonoclonal antibodies reacted with all salmonella species bearing O_4 antigen. |
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| Keywords: | monoclonal antibodies salmonella speciesl O_4 and O_ 4antigens |
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