| 一种新的藻红蛋白的亚基组成分析 |
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| 引用本文： | 王盛,钟伏弟,吴祖建,林奇英,谢联辉.一种新的藻红蛋白的亚基组成分析[J].福建农林大学学报(自然科学版),2004,33(1):68-71. |
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| 作者姓名： | 王盛 钟伏弟 吴祖建 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所,福建,福州,350002 |
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| 基金项目： | 福建省科技厅重大资助项目(2000H004、2001H127). |
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| 摘 要： | 经盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析等分离纯化,从珊瑚藻中分离纯化出R-藻红蛋白(R-PE).对其分子质量和亚基组成进行研究,结果表明:R-PE的分子质量为194ku,而目前报道的R-藻红蛋白分子质量为240ku;α、β亚基分子质量均为20ku左右,γ亚基分子质量约为31ku,推测的亚基组成为(αβ)4γ不同于其他红藻中(αβ)6γ的藻红蛋白组成].
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| 关 键 词： | 珊瑚藻 藻红蛋白 亚基组成 |
| 文章编号： | 1006-7817(2004)01-0068-04 |
| 修稿时间： | 2002年6月20日 |
Analysis on subunit composition of a new phycoerythrin |
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| WANG Sheng,ZHONG Fu-di,WU Zu-jian,LIN Qi-ying,XIE Lian-hui.Analysis on subunit composition of a new phycoerythrin[J].Journal of Fujian Agricultural and Forestry University,2004,33(1):68-71. |
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| Authors: | WANG Sheng ZHONG Fu-di WU Zu-jian LIN Qi-ying XIE Lian-hui |
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| Abstract: | By using fraction precipitation methods such as ammonium sulfate, ion exchanging, modified hydrorylapatite and gel filtration chromatography, a kind of phycoerythrin (R-PE) from Corallina officinalis was purified. The results of physical and chemical characteristics showed that the molecular mass of R-PE was 194 ku, which had been reported to be 240 ku; the molecular masses of α and β subunits were about 20 ku; the molecular mass of γ subunit was about 31 ku; subunit composition was not (αβ)_(6γ) (in other kinds of phycoerythrins) but (αβ)_(4γ) presumably. |
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| Keywords: | Corallina officinalis phycoerythrin subunit composition |
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