小麦品种资源耐盐性鉴定

目的研究TaERECTA基因超表达后对小麦耐盐性的影响。方法以转TaERECTA基因小麦为材料，分别在芽期和苗期高盐胁迫，测定种子发芽势、发芽率、发芽指数和相对盐害指数，以及植株枯萎数、幼苗抗氧化酶活性和丙二醛(MDA)含量。结果芽期盐胁迫下，与野生型(WT)株系相比，TaERECTA转基因小麦种子发芽势、发芽率和发芽指数较高，相对盐害指数较低(P<0.05)；苗期盐胁迫下，转基因小麦幼苗平均枯萎数较低，抗氧化酶活性较高，且SOD活性显著高于CK株系(P<0.05)。结论一定盐胁迫下，TaERECTA基因超表达可以增强小麦耐盐性，其作用机制可能是TaERECTA激活组织细胞SOD酶活性，其他抗氧化酶相互协同减轻盐胁迫伤害。转基因株系L10相对盐害指数较小，综合抗盐能力较强，可作为下一步深入研究TaERECTA调控小麦耐盐性功能的重要材料。

The receptor-like cytoplasmic kinase STRK1 phosphorylates and activates CatC,thereby regulating H2O2 homeostasis and improving salt tolerance in rice

PurposeTo investigate the influence of TaERECTA overexpression on salt tolerance of bread wheat.MethodTaERECTA genetically modified (GM) wheat was used to identify the germination energy, germination rate, germination index and relative salt stress index (RSSI) of seeds, and plant wilting numbers, antioxidant enzyme activity and MDA content of fresh seedlings.ResultCompared to control, the seeds of TaERECTA GM plants displayed higher germination energy, germination rate and germination index, whereas lower RSSI under salt stress at germination stage; fresh seedlings of GM plants showed lower plant wilting numbers and higher antioxidant enzyme activity, with significantly higher SOD activity as to control plants (P<0.05).ConclusionThese results suggest that TaERECTA overexpression could improve salt tolerance of bread wheat after salt stress, due to the mechanism of TaERECTA promotion on the SOD activity of organelle, and other antioxidant enzymes synergistically reduce salt stress injury. Transgenic wheat lines L10 showed lower RSSI and integrally stronger ability of salt tolerance, and line L10 can be used as an important material for further investigation of TaERECTA function on salt tolerance of bread wheat.