| 细菌DNA紫外线辐射损伤和DNA修复系统的实验探究活动 |
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| 引用本文： | 姚波, 何鹏飞, 黄敏, 等. 玉米顶腐病病原细菌成团泛菌C3菌株对不同环境适应性的研究[J]. 云南农业大学学报（自然科学）, 2019, 34(2): 210-215. DOI: 10.12101/j.issn.1004-390X(n).201803022 |
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| 作者姓名： | 姚波 何鹏飞 黄敏 吴毅歆 李兴玉 何月秋 |
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| 作者单位： | 1.云南农业大学 植物保护学院，云南 昆明 650201;2.微生物菌种筛选与应用国家地方联合工程研究中心，云南 昆明 650217 |
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| 基金项目： | 云南省玉米产业体系（2017KJTX002） |
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| 摘 要： | 目的探究玉米顶腐病病原细菌——成团泛菌(Pantoea agglomerans) C3菌株对不同环境的适应性。方法通过稀释涂板法测定成团泛菌在不同温度、水质和寄主植物中的存活动态。结果C3菌株对紫外线辐射较为敏感，照射40 s后致死率达到100%；相较于37 ℃，4 ℃和20 ℃有利于C3菌株生存，灭菌自来水、水沟水和自来水中的菌体数量高于臭水沟水和池塘水；另外，C3菌株可在多种植物叶片上定殖，定殖密度表现为玉米>烟草>棉花>三叶草>稗草。结论成团泛菌具有很强的环境适应性，能够在不同的环境中进行生存和繁殖。
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| 关 键 词： | 成团泛菌 紫外线 环境适应性 定殖 |
| 收稿时间： | 2018-03-14 |
| 修稿时间： | 2018-09-25 |
Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice |
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| Bo YAO, Pengfei HE, Min HUANG, et al. A Study on the Environmental Adaptation of Pantoea agglomerans C3 Strain, a Causal Agent of Maize Top Rot Disease[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(2): 210-215. DOI: 10.12101/j.issn.1004-390X(n).201803022 |
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| Authors: | Bo YAO Pengfei HE Min HUANG Yixin WU Xingyu LI Yueqiu HE |
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| Affiliation: | 1.College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;2.National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217, China |
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| Abstract: | PurposeTo investigate the environmental adaptation of Pantoea agglomrans C3 causing maize top rot.MethodIts survival dynamic was detected in different temperatures, surface water of different quality and host plants with dilution plate method.Result C3 was sensitive to ultraviolet radiation (UVC), and all of its cells could be killed after 40 seconds radiation. Compared with 37 ℃, 4 ℃ and 20 ℃ facilitated this plant pathogen’s survival. The number of C3 in sterilized tap water, ditch water and tap water were more than those in drainage water and pond water. In addition, C3 could colonize in various plant leaves, where the density order from high to low was maize, tobacco, cotton, clover and barnyard grass.ConclusionP. agglomerans has strong environmental adaptability and can survive and reproduce in different environments. |
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| Keywords: | Pantoea agglomerans ultraviolet radiation (UVC) environmental adaptation colonization |
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