不同玉米品种玉米蚜田间消长与其主要生化物质的相关性研究

目的明确玉米蚜吸食抗、感玉米品种后对叶片内抗坏血酸(AsA)和谷胱甘肽(GSH)含量、抗坏血酸过氧化物酶(APX)活性及活性氧代谢的影响。方法测定被玉米蚜吸食24、48和96 h后感蚜品种蠡玉 16和抗蚜品种郑单 958叶片中AsA、GSH、O₂⁻、H₂O₂和OH⁻的含量以及APX活性。结果被玉米蚜刺吸24、48和96 h后，蠡玉 16玉米幼苗叶片中的AsA含量与对照相比显著增高(P<0.05)；被玉米蚜吸食48和96 h后，郑单 958幼苗叶片内的AsA含量显著高于对照(P<0.05)；被玉米蚜吸食24 h后，郑单 958幼苗叶片中的GSH含量显著低于对照(P<0.05)，被玉米蚜吸食48 和96 h后，蠡玉 16和郑单 958幼苗叶片中的GSH含量均显著高于对照(P<0.05)；随着吸食时间的增加，蠡玉 16和郑单 958叶片中的APX活性逐渐降低；被玉米蚜取食24、48 和96 h后，蠡玉 16幼苗中的H₂O₂含量均显著高于对照(P<0.05)；而被玉米蚜吸食24 h后，郑单 958叶片中的H₂O₂含量与对照差异不显著(P>0.05)，但在48 和96 h后显著高于对照(P<0.05)；被玉米蚜吸食48和96 h后，蠡玉 16幼苗叶片中的OH⁻含量高于对照，而在24、48和96 h后郑单 958幼苗叶片中的OH⁻含量均高于对照；被蚜虫吸食48和96 h后，郑单 958幼苗叶片中的O₂⁻含量高于对照，而在24、48和96 h后蠡玉 16幼苗叶片中的O₂⁻含量均高于对照。结论被玉米蚜吸食后，抗蚜和感蚜品种叶片中的AsA、GSH、APX含量及活性氧代谢均发生相应的变化，对感蚜品种蠡玉 16的活性氧影响较大，对抗蚜品种郑单 958影响较小。

Aphis immunity of teosinte-corn hybrids

PurposeIn order to determine the effect of maize aphid pricking stress on the contents of AsA and GSH, APX activity and active oxygen metabolism in the leaves of resistant and susceptible maize varieties.MethodThe contents of AsA,GSH,O₂⁻, H₂O₂ and OH⁻ and APX activity in the leaves of susceptible maize aphid cultivar Liyu16 and resistant maize aphid cultivar Zhengdan958 were determined at 24, 48 and 96 hours under maize aphid pricking stress.ResultThe content of AsA in the leaves of Liyu16 maize seedlings increased significantly at 24, 48 and 96 h, while that in Zhengdan958 maize seedlings at 48 and 96 h was significantly higher than that in the control (P<0.05), and the contents of GSH in leaves of Zhengdan958 seedlings were significantly lower than that of the control at 24 h (P<0.05). The contents of GSH in the leaves of Liyu16 and Zhengdan958 seedlings were significantly higher than that of the control at 48 and 96 h of pricking (P<0.05); APX activities in the leaves of Liyu16 and Zhengdan958 varieties decreased gradually with the prolongation of aphid pricking time; the contents of H₂O₂ in the leaves of Liyu16 varieties were significantly higher at 24, 48 and 96 h under maize aphid pricking stress (P<0.05), but at 24 h under maize aphid pricking stress, the contents of H₂O₂ in the leaves of Zhengdan958 varieties were significantly higher than that of the control (P<0.05). The contents of OH⁻ in the leaves of Liyu16 seedlings were significantly higher than those of the control at 48 h and 96 h of pricking (P<0.05), while the content of OH⁻ in leaves of Zhengdan958 were significantly higher at 24, 48 and 96 h under maize aphid pricking stress(P<0.05). The contents of O₂⁻ in the leaves of Zhengdan958 seedlings were significantly higher than those of the control at 48 h and 96 h of pricking (P<0.05), while the content of O₂⁻ in the leaves of Liyu16 were significantly higher at 24, 48 and 96 h of maize aphid pricking stress (P<0.05).ConclusionThe above results indicated that the contents of AsA, GSH, APX and the metabolism of reactive oxygen species in the leaves of aphid-resistant and susceptible cultivars changed correspondingly when they were susceptible to maize aphid pricking stress, but the effects on reactive oxygen species of susceptible cultivar Liyu16 were greater than those on resistant cultivar Zhengdan958.