蔬菜根结线虫致病机理及其防控技术研究进展

目的研究放线菌菌株1331的最佳发酵工艺条件，并探究其发酵液对南方根结线虫2龄幼虫的抑杀作用。方法在摇瓶试验中采用单因素筛选和正交试验对其发酵条件进行优化。结果该菌株的最佳摇瓶发酵培养基为发酵培养基Ⅱ；最佳发酵条件为装液量80 mL (500 mL摇瓶)，种子液接种量为5% (体积分数)，初始pH值7.0，培养温度25 ℃，150 r/min培养7 d。该条件下菌株1331产孢量为1.68×10⁹ CFU/mL，其5%发酵稀释液处理南方根结线虫卵9 d，卵孵化率与对照相比降低了70.59%；10%和20%发酵稀释液对南方根结线虫2龄幼虫48 h的校正死亡率分别达88.97%和100%。结论放线菌菌株1331发酵液可有效抑杀南方根结线虫，具有潜在生防价值。

Resistance to root-knot nematodes Meloidogyne spp. in woody plants

PurposeIn order to study the optimization of fermentation conditions and explore toxicity effects of 1331 fermented fluid to the second-stage juveniles of Meloidogyne incognita (Mi J2).MethodSingle-factor and orthogonal test were used in shaking flasks to optimize the fermentation conditions of strain 1331.ResultThe optimum fermentation culture medium was fermentation mediumⅡ. And the optimal fermentation condition was as follows: medium volume 80 mL in 500 mL shaking flask, inoculation size of 5% (volume fraction), initial pH 7.0, culture temperature 25 ℃, shaking at 150 r/min for 7 days. Under this condition, the sporulation of strain 1331 was 1.68×10⁹ CFU/mL; compared with the control, the hatching rate of Mi J2 was lowered down by 70.59% by dealing with 5% 1331 supernatant fermented fluid 9 d, and the corrected mortality rate of Mi J2 was 88.97% and 100% by dealing with 10% and 20% 1331 supernatant fermented fluid for 48 h.ConclusionThe strain 1331 fermented fluid had toxicity to Mi J2.