高加索三叶草、白三叶及其杂种F1代的RAPD分析

目的研究LeNCED1基因对白三叶水分利用效率的影响，为白三叶的节水抗旱提供一定的理论指导。方法分别以7株含转SAG₁₃-LeNCED1基因白三叶和非转基因白三叶的老叶和嫩叶为材料，研究其ABA的含量、光合特性、叶绿素含量及水分利用效率。结果转SAG₁₃-LeNCED1基因显著提高了白三叶老叶的ABA含量(P<0.05)，却显著降低了白三叶老叶的叶绿素b、胞间CO₂浓度、蒸腾速率和气孔导度(P< 0.05)。转LeNCED1基因显著降低了白三叶嫩叶叶绿素a、叶绿素b、净光合速率和蒸腾速率(P<0.05)，却显著提高了白三叶嫩叶的胞间CO₂浓度和气孔导度(P<0.05)。结论转入LeNCED1基因能显著提高白三叶的长期水分利用效率(P<0.05)。

Over-expression of LeNCED1 in tomato (Solanum lycopersicum L.) with the rbcS3C promoter allows recovery of lines that accumulate very high levels of abscisic acid and exhibit severe phenotypes

PurposeThe effect of the LeNCED1 gene on the water use efficiency of white clover was studied to provide a certain theoretical guidance for its water saving and drought resistance.MethodWe studied the ABA content, photosynthetic characteristics, pigment and water use efficiency of the old and young leaves in the transgenic (LeNCED1) plants with the SAG₁₃ promoter and non-transgenic white clover.ResultThe LeNCED1 gene could significantly increase the ABA content in the old leaves of white clover (P<0.05), but reduced significantly chlorophyll b, intercellular CO₂ concentration, transpiration rate and stomatal conductance in the old leaves of white clover (P<0.05). What’s more, the LeNCED1 gene decreased the content of chlorophyll a, chlorophyll b, net photosynthesis rate and transpiration rate in young leaves of white clover (P<0.05), but significantly increased the intercellular CO₂ concentration and stomatal conductance of young leaves of white clover (P<0.05).ConclusionThe long-term water use efficiency of white clover was improved by transferring LeNCED1.