大麻哈鱼繁殖特征及呼玛河原始产卵场生境功能验证

大麻哈鱼 (Oncorhynchus keta) 是重要的洄游性经济鱼类，在中国分布于黑龙江、乌苏里江、绥芬河和图们江水域。为保护大麻哈鱼种质资源，恢复图们江流域野外种群数量，该研究基于微卫星标记技术，利用10对微卫星标记，对355尾回捕子代和91尾繁殖亲本开展了亲子关系鉴定研究。结果表明，检测等位基因数 (N_a) 201个，观测杂合度 (H_o) 为0.617~0.895，平均0.744；期望杂合度 (H_e) 为0.676~0.909，平均0.824；多态信息含量 (PIC) 为0.628~0.900，平均0.805。利用Cervus 3.0软件计算10个微卫星座位的累积非亲排除率 (CEP) 达99.95%。5个回捕样本与繁殖亲本存在亲子关系，确定为增殖放流的大麻哈鱼。该研究成功利用微卫星标记技术明确回捕大麻哈鱼归属地，为巩固中国鱼源国地位提供有力证据，同时为评估大麻哈鱼的增殖放流效果提供技术支撑。

Underyearling pink and chum salmon in the western Bering Sea during the fall season (September and October 2013): distribution,feeding, habits,and growth patterns

Chum salmon (Oncorhynchus keta), an important migratory economic fish, only recorded in the Amur, Wusuli, Suifen Current (Heilongjiang Province) and Tumen River (Jilin Province) in China. In order to protect the germplasm resources and increase their stocks in the wild, ten pairs of polymorphic microsatellite markers were used to identify the genetic relationship of 355 recaptured individuals and 91 breeding parents. A total of 201 alleles were obtained. The observed and expected heterozygosity were 0.617–0.895 and 0.676–0.909, respectively. The polymorphic information content (PIC) ranged from 0.628 to 0.900, with a mean of 0.805. The combined non-exclusion probability (first parent) of the ten loci was 99.95%. The results show that five of the 355 recaptured individuals were identified as the offsprings of the breeding parents in Tumen River. In this study, microsatellite marker technology was successfully used to determine the location of salmon, which provides strong evidence for consolidating the status of China's fish source country and technical support for evaluating the effect of O. keta breeding and releasing.