三七根腐病研究进展

ttttt目的ttttt明确臭氧处理连作土壤后对三七出苗和生长的影响，以及对三七自毒皂苷的降解和对根腐病菌的抑制作用。ttttttttttttt方法ttttt用不同质量浓度的臭氧水处理三七连作土壤，调查不同处理对三七种子出苗和农艺性状的影响；用高效液相色谱法研究臭氧对自毒皂苷R₁、Rg₁、Re、Rd和Rg₂的降解作用；用平板计数法研究臭氧对主要根腐病菌茄腐镰刀菌(Fusarium solani)和毁灭柱孢菌(Cylindrocarpon destructans)的杀灭作用。tttttttttttttttttt结果ttttt利用0.3~0.6 mg/L臭氧水处理连作土可显著提高三七种子的出苗率和生长势，但不同质量浓度及水量的处理间三七幼苗的株高、整株鲜重和地下部干重等农艺性状指标不存在显著差异。臭氧可以降解三七连作土壤中主要的皂苷类自毒物质，抑制引起三七根腐病的主要病原菌茄腐镰刀菌和毁灭柱孢菌的生长。ttttttttttttt结论ttttt臭氧处理可以通过降解自毒皂苷抑制三七主要根腐病菌，从而促进连作土壤中三七的出苗和生长。tttt

Autotoxic potential in soil sickness: a re-examination

tttttPurposetttttTo determine the effects of ozone treatment on the germination and growth of Panax notoginseng in continuous cropping soil and the degradation of autotoxic ginsenosides and the inhibitory of root rot pathogens.ttttttttttttttttttMethodstttttThe effects of different mass concentrations of ozone water on the seed germination and growth of P. notoginseng in continuous cropping soil were investigated. The degradation of autotoxic ginsenosides R₁, Rg₁, Re, Rd and Rg₂ by ozone was studied by HPLC. The inhibition effect of ozone on the main root rot pathogens was studied by colony counting method.ttttttttttttttttttResultstttttWhen the concentration of ozone water ranged from 0.3 to 0.6 mg/L, the seed germination and plant growth were significantly increased in continuous cropping soil, but there were no significance in the plant weight, fresh weight and root dry weight among different concentrations and water does treatments. Ozone could significantly degrade the main autotoxic ginsenosides, inhibit the growth of root rot pathogens Fusarium solani and Cylindrocarpon destructans.ttttttttttttttttttConclusiontttttOzone water treatment can significantly increase the seed germination and plant growth of P. notoginseng in continuous cropping soil by degrading the main autotoxic ginsenosides and inhibiting the major root rot pathogens.ttttttttt