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Trial of a novel experimental Toxoplasma iscom vaccine in pregnant sheep
Authors:D Buxton  A Uggla  K L?vgren  K Thomson  A Lundén  B Morein  D A Blewett
Affiliation:1. Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH, Scotland;2. Swedish University of Agricultural Science, S-75007 Uppsala, Sweden;3. National Veterinary Institute, S-75007, Uppsala, Sweden;1. Sheep Veterinary Practice Strobel, Am Hopfenberg 8, 89352 Stoffenried, Germany;2. Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany;3. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Suedufer 10, 17493 Greifswald – Riems, Germany;4. Clinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-University Munich, Sonnenstr. 16, 85764 Oberschleissheim, Germany;5. State Veterinary Investigation Centre Aulendorf, Loewenbreitestr. 20, 88326 Aulendorf, Germany;6. Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine, Hannover, Foundation, Buenteweg 2, 30559 Hannover, Germany;1. Australian Animal Health Laboratory, CSIRO-CAFHS, 5 Portarlington Road, Geelong, Victoria, Australia;2. National Centres for Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba, Canada;1. CSIRO-Australian Animal Health Laboratory, 5 Portarlington Rd, Geelong, Australia;2. Canadian Food Inspection Agency, National Centre for Foreign Animal Diseases, 1015 Arlington St, Winnipeg, Canada;3. Wageningen Bioveterinary Research, Department of Bacteriology and Epidemiology, Houtribweg 39, 8221RA Lelystad, The Netherlands;1. Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA;2. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA;3. ISaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13005 Ciudad Real, Spain;1. Department of General and Oncologic Surgery, University of Perugia, Perugia, Italy;2. Department of General and Oncologic Surgery, University of Perugia, Terni, Italy;3. Department of Surgical Sciences, Sapienza University of Rome, Italy;4. Department of General Surgery, Saint Mary Hospital, University of Perugia, Terni, Italy;5. Department of Digestive Surgery, St. Maria Hospital, Terni, Italy
Abstract:Fifteen vaccinated ewes (group 1) and 13 unvaccinated ewes (group 2) were each challenged orally with 2000 sporulated Toxoplasma gondii oocysts at 91 (+/- 1) days' gestation. Another four pregnant ewes acted as unvaccinated unchallenged controls. Lamb mortality in group 1 was 36.4% after a mean gestation of 141 days while in group 2 it was 64.7% after a mean gestation of 131.5 days. These differences were not statistically significant. However, substantially more specific antibody was detected in precolostral sera from live lambs from vaccinated ewes than in live lambs from unvaccinated ewes in group 2.
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