木二糖和木三糖的分离及其用于双歧杆菌的体外培养

采用凝胶过滤层析技术获得了高纯度木二糖和木三糖,并研究了它们在体外对青春双歧杆菌的增殖效果.以聚丙烯酰胺凝胶(Bio-Gel P-2)为层析介质,采用快速蛋白液相层析(FPLC)系统为平台,以脱气高纯水洗脱,制备分离了木二糖和木三糖,并分别以它们为碳源,研究了体外增殖培养青春双歧杆菌的生长历程.结果表明,体外培养48 h后,木糖、木二糖和木三糖分别将青春双歧杆菌菌体浓度从0.10 g/L增殖至0.16、0.33和0.47 g/L,木三糖对青春双歧杆菌的增殖效果最好,木二糖次之,木糖的增殖效果不明显.木二糖和木三糖在体外培养青春双歧杆菌48 h后,能分别将体系的pH值从7.0降到4.40和4.42.

PREPARATIVE SEPARATION OF XYLOBIOSE AND XYLOTRIOSE AND THEIR IN VITRO APPLICATION IN FERMENTATION BY BIFIDOBACTERIA

Xylobiose and xylotriose were prepared from xylooligosaccharides by gel permeation chromatography,and their in vitro cultivation properties by Bifidobacterium adolescentis were investigated.Xylobiose and xylotriose were separated on polyacrylamide gel Bio-Gel P-2 by FPLC system with degased high-purified water as eluant,and their (biological) characteristics toward bifidobacteria were studied.The results showed that the cell-dry-weights of B.adolescentis were 0.16,0.33 and 0.47 g/L,respectively,when xylose,xylobiose and xylotriose were used as carbon souces after 48 h cultivation in vitro.Xylotriose had superior effect than xylobiose and xylose on proliferation of B.adolescentis,and xylobiose had better effects than xylose.Xylobiose and xylotriose can reduce pH values of medium from 7.0 to 4.40 and 4.42,respectively.