The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat |
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Authors: | PK Gupta RK Varshney |
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Institution: | (1) Biochemical Sciences Division, National Chemical Laboratory, Pune, India;(2) Pulses Research Station, Mahatma Phule Krishi Vidyapeeth, Rahuri, India;(3) Biotechnology Department, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India; |
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Abstract: | In recent years, a variety of molecular markers, based on microsatellites or simple sequence repeats (SSRs) have become the
markers of choice, thus necessitating their development and use in a variety of plant systems. In this review, the basic principles
underlying different hybridization-based (oligonucleotide fingerprinting) and PCR based approaches (STMS, MP-PCR, AMP-PCR/
ISSR/ ASSR, RAMPs/ dRAMPs, SAMPL), making use of microsatellites, have been outlined. Different methods for enrichment of
genomic libraries for microsatellites have also been outlined. Relevant literature on the subject, giving a summary of results
obtained using each approach, has been reviewed and critically discussed. The review also includes a discussion on literature,
which deals with the use of microsatellites in genome mapping, gene tagging, DNA fingerprinting, characterization of germplasm
and cytogenetics research. Special emphasis has been laid on the genome of bread wheat, where the work done in the authors'
own laboratory has also been briefly reviewed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | microsatellites molecular markers SSRs |
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