| RAPD技术及其在动物医学中的应用 |
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| 引用本文： | 李淑梅,汪铭书,程安春.RAPD技术及其在动物医学中的应用[J].动物医学进展,2003,24(3):22-24,57. |
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| 作者姓名： | 李淑梅 汪铭书 程安春 |
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| 作者单位： | 四川农业大学动物科技学院,四川,雅安,625014 |
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| 摘 要： | RAPD技术即随机扩增多态性DNA技术，是一种建立在PCR基础上的新的DNA多态性检测技术，其主要特点是利用随机寡核苷酸引物扩增基因组DNA片段，通过凝胶电泳分析其指纹图谱特征。该技术具有快速灵敏，程序简便等优点，现已经广泛应用于多个领域，此文综述了RAPD技术的原理、优缺点、影响因素及其在动物医学中的应用。
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| 关 键 词： | RAPD技术 动物医学 随机扩增多态性 DNA技术 凝胶电泳分析 指纹图谱特征 |
| 文章编号： | 1007-5038(2003)03-0022-03 |
RAPD Technique and its Application in Animal Medicine |
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| LI Shu mei,WANG Ming shu,CHENG An chun.RAPD Technique and its Application in Animal Medicine[J].Progress In Veterinary Medicine,2003,24(3):22-24,57. |
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| Authors: | LI Shu mei WANG Ming shu CHENG An chun |
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| Abstract: | RAPD (Random Amplify Polymorphism DNA) is a new technique based on PCR(polymerase chain reaction) method for the examination of DNA sequence Polymorphism and establishment of molecular markers. For its simple manipulation and efficiency in providing abundant and informative data on DNA sequence polymorphism of many loci,the technique has been widely applied as a molecule marker in many fields. In this article, many aspects of RAPD, such as its principle, advantages and disadvantages, influence factors and application in animal medicine are reviewed. |
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| Keywords: | RAPD principle influence factors application |
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