| 橡胶树胶乳全蛋白的提取及双向电泳分析 |
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| 引用本文： | 徐智娟,袁坤,丁璇,杨礼富. 橡胶树胶乳全蛋白的提取及双向电泳分析[J]. 热带作物学报, 2010, 30(4): 1-4 |
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| 作者姓名： | 徐智娟 袁坤 丁璇 杨礼富 |
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| 作者单位： | 1 中国热带农业科学院橡胶研究所/农业部橡胶树生物学重点开放实验室 海南儋州 571737;2 海南大学农学院 海南儋州 571737;中国热带农业科学院橡胶研究所/农业部橡胶树生物学重点开放实验室 海南儋州 571737;海南大学农学院 海南儋州 571737;中国热带农业科学院橡胶研究所/农业部橡胶树生物学重点开放实验室 海南儋州 571737 |
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| 摘 要： | 以橡胶树无性系RRIM600为材料,采用TCA(三氯乙酸)-丙酮沉淀法提取胶乳全蛋白,17 cm pH 5～8固相pH梯度预制干胶条和快速银染法对提取的蛋白进行分离和染色,所得到的电泳图谱背景清晰,横条纹和竖条纹少,且蛋白点数量多。通过本研究获得了高分辨率的胶乳全蛋白双向电泳(2-DE)图谱的方法,为进一步开展橡胶树胶乳全蛋白质组学研究奠定了基础。
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| 关 键 词： | 橡胶树 胶乳 全蛋白 双向电泳 |
The extration and two-dimensional electrophoresis analysis of total proteins from the rubber tree latex |
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| Affiliation: | 1 Rubber Research Institute, CATAS and Key Laboratory of Rubber Biology, Ministry of Agriculture, Danzhou, Hainan 571737; 2 College of Agronomy, Hainan University, Danzhou, Hainan 571737;Rubber Research Institute, CATAS and Key Laboratory of Rubber Biology, Ministry of Agriculture, Danzhou, Hainan 571737;College of Agronomy, Hainan University, Danzhou, Hainan 571737;Rubber Research Institute, CATAS and Key Laboratory of Rubber Biology, Ministry of Agriculture, Danzhou, Hainan 571737 |
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| Abstract: | Total proteins of rubber tree latex were extracted from Hevea brasiliensis clone RRIM600 by improved TCA-acetone precipitation, and 17cm pH 5-8 IPG strip and silver nitrogen was applied to separate and stain rubber tree latex proteins. The results showed that the protein pattern of clear gel background , little horizontal and vertical stripes and relatively more protein spots was achieved. Exploring the strategy to gain high resolution 2-DE profile of the total protein from latex by the research, which provides way for further study of total proteome from rubber tree latex. |
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| Keywords: | rubber tree (Hevea brasiliensis) latex the total protein 2-DE |
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