| 海南滨海椰林Bt菌株的筛选与鉴定 |
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| 引用本文： | 孙晓东,李朝绪,吕朝军,钟宝珠,覃伟权.海南滨海椰林Bt菌株的筛选与鉴定[J].热带作物学报,2011,32(11):2129-2132. |
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| 作者姓名： | 孙晓东 李朝绪 吕朝军 钟宝珠 覃伟权 |
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| 作者单位： | 中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所 |
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| 基金项目： | 海南省自然科学基金（No. 310103）； 公益性行业（农业）科研专项项目（No. 200903026 ）。 |
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| 摘 要： | 以海南省文昌市的滨海椰林为试点,采集144份天然土壤样品,分离出10株苏云金芽孢杆菌,对其中10株菌株进行光学显微镜观察,发现菌体有长菱形、短菱形、方形等晶体类型.对这10株菌进行cry1、cry11、cry2、cry3、cry4、cry5、cry6、cry7、cry8、cry9、cry10类基因的PCR-RFLP鉴定...
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| 关 键 词： | 苏云金芽孢杆菌 cry基因鉴定 PCR-RFLP |
Isolation and Identification of Bacillus thuringiensis Strains from Coconut Grove of Seaside, Hainan Province |
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| SUN Xiaodong,LI Chaoxu,LV Chaojun,ZHONG Baozhu and QIN Weiquan.Isolation and Identification of Bacillus thuringiensis Strains from Coconut Grove of Seaside, Hainan Province[J].Chinese Journal of Tropical Crops,2011,32(11):2129-2132. |
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| Authors: | SUN Xiaodong LI Chaoxu LV Chaojun ZHONG Baozhu and QIN Weiquan |
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| Institution: | Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | One hundred and forty - four soil samples were collected from coconut crove of seaside, Hainan Province, and 10 Bacillus thuringiensis isolates were obtained. We found there were long bipyramidal crystal, short bipyramidal crystal, square crystal types in the 10 Bt isolates. Comparison of plasmid DNA patterns by agarose gels electrophoresis was used to discriminate the types of the isolates, and the diversity of these isolates was prominent. cry1, cry1I and cry2 type genes were found in these isolates by PCR-RFLP method, but the cry gene-types of three isolates BH10, BH17, and BH21 were not known. SDS-PAGE results showed that the 10 wild isolates could express Cry proteins with 130 kD molecular mass, and 2 isolates could express both 130 kD and 60 kD protein. Bioassay results of 10 isolates indicated that six of them were toxic to Brontispa longissina. |
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