植物叶绿体蛋白质组学研究进展

亚细胞蛋白质组学研究是近年来蛋白质组学研究中的一个热点,蛋白质组分析已成为在亚细胞水平鉴定植物功能蛋白的有力工具.叶绿体作为重要的植物细胞器,在植物蛋白质组学中已有较多的研究.随着双向电泳技术的改进和质谱灵敏度的提高,并结合不断增多的拟南芥、水稻、玉米等植物的数据库信息相结合,许多植物叶绿体中的蛋白质已经被成功鉴定.叶...

Research Progress on Plant Chloroplast Proteomics

Subcellular proteomics is a hotspot in the study of proteomics in recent years, and proteomic analysis is a powerful approach to identify plant functional proteins. As an important plant organelle, chloroplast has been intensively studied in plant proteomics. With the sensitivity improvement of two-dimensional electrophoresis and mass spectrometry, and combining with the in- creasing database information from Arabidopsis, rice, corn and other plants, many chloroplast proteins can be quickly identified. Compared with other subcellular proteomics, chloroplast proteome is more comprehensive. Sub-cell separation system, together with two-dimensional electrophoresis that identify the protein components of chloroplast proteins is a useful method to obtain information of chloroplast proteins and to identify the function. This paper mainly describes the development of proteomics and proteomics technologies, and gives a review of chloroplast proteins in order to fully understand the composition and character of chloroplast proteins, and their role in chloroplast metabolic network.