| 两个牡丹杂交系种子胚培养技术研究 |
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| 引用本文： | 曾端香,袁 涛,王莲英,王 福.两个牡丹杂交系种子胚培养技术研究[J].热带作物学报,2011,31(3):8-12. |
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| 作者姓名： | 曾端香 袁 涛 王莲英 王 福 |
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| 作者单位： | 1 国家林业局管理干部学院 北京 102600；2 北京林业大学园林学院 北京 100083;2 北京林业大学园林学院 北京 100083;2 北京林业大学园林学院 北京 100083;3 牡丹芍药科技开发有限公司 河南洛阳 471500 |
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| 摘 要： | 为保存和扩繁牡丹杂交系种质资源，提供牡丹组培苗的菌根化供试体系，本试验筛选牡丹杂交系‘Z1-10-3’和‘Z1-WM’的种子，取种胚进行胚培养，建立了牡丹胚培养无菌体系。结果表明：成熟种子经70%酒精2 min与0.1%HgCl 20 min表面消毒后去外种皮切开胚乳直接取胚接种，3 d时启动率达76.7%，比直接用种子和去外种皮的种子接种启动时间提前30～50 d。胚根苗直接培养的最佳培养基和培养条件为：1/2MS＋IBA 1.0 mg/L ＋AC 0.4 g/L＋Sugar 30 g/L，在自然弱光照下培养。
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| 关 键 词： | 牡丹 杂交系种子 胚培养 最佳培养基 |
Embryo-culture of 2 Hybrid Strains Seeds of Tree Peony |
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| Institution: | 1 State Academy of Forestry Administration, Beijing 102600, China; 2 College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;2 College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;2 College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;3 Peony Technology Development Co. Ltd., Luoyang, Henan 471500, China |
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| Abstract: | In order to conserve and multiply germplasm resources of hybrid strain of tree peony, and provide tissue culture seedling with arbuscular mycorrhizae, the germ-free system of embryo-culture was constructed in hybrid seeds of tree peony('Z1-10-3','Z1-WM').Firstly, the seed surface of tree peony were sterilized by 70% alcohol 2 min and 0.1% HgCl2 20 min. Secondly, the seed coat and endosperm were wiped off. Then the embryo was used to cultivate. About 76.7% of embryos were germinated after 3 d. The starting time of growth of explants with embryo taken directly was 30-50 d prior to the seeds with exopleura. Results showed that the best media and cultivation condition of radicle plantlet was 1/2MS+ IBA 1.0 mg/L+AC 0.4 g/L+Sugar30 g/L with natural low light. |
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| Keywords: | tree peony hybrid seeds embryo-culture optimal media |
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