秀珍菇菌种复壮方法比较

以从秀珍菇(Pleurotus geesteranus)污染菌包中分离得到的3个秀珍菇菌株为出发菌株,采用挑取尖端菌丝、基内菌丝及气生菌丝3种方法进行复壮处理,共获得处理菌株8个。以出发菌株为对照,比较处理菌株的菌落形态、菌丝生长速度及菌丝生物量,并通过出菇实验以及dsRNA检测,对不同复壮方法的复壮效果进行评价。结果表明:尖端菌丝和基内菌丝挑取法能显著提高出发菌株的菌丝生长速度和生物量,可以缩短出菇周期并提高出菇产量;而气生菌丝挑取法则不能对出发菌株进行复壮,其菌丝生物量和出菇产量反而比出发菌株有所降低。

Comparative Study on Methods of Spawn Rejuvenation of Pleurotus geesteranus

Three starting strains were separated from contaminated cultivating bags of Pleurotus geesteranus. Eight processed strains of P. geesteranus were taken through picking tip mycelia, substrate mycelia and aerial mycelia. Compared with the starting strains, the colonial morphology, growing speed and biomass were studied. After the fruiting experiment and dsRNA technology test, the rejuvenation effect of different methods was evaluated. This studies showed picking tip mycelia and substrate mycelia could obviously improve the growth speed and the biomass of the mycelia, shorten the fruiting cycle and increase the production while picking aerial mycelia could not rejuvenate the starting strains but comparatively reduce the biomass of the mycelia and the mushroom production.