| 一株对红棕象甲幼虫和卵有致病力的病原菌的分离鉴定 |
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| 引用本文： | 张晶,覃伟权,彭正强,阎伟.一株对红棕象甲幼虫和卵有致病力的病原菌的分离鉴定[J].热带作物学报,2011,32(12):2331-2335. |
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| 作者姓名： | 张晶 覃伟权 彭正强 阎伟 |
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| 作者单位： | 1海南大学环境与植物保护学院
2中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所;中国热带农业科学院椰子研究所;中国热带农业科学院环境与植物保护研究所 |
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| 基金项目： | 国家科技支撑计划（No. 2007BAD48B01，No. 2007BAD48B06）；农业部公益性行业科研专项（No. 200903026）；海南省自然科学基金（No. 310102）；中央级公益性科研院所基本科研业务费专项（No. 2011hzs1J013，No. YWFZX2010 -13（N））；海南大学'211工程'建设项目；海南大学环境与植物保护学院研究生创新平台。 |
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| 摘 要： | 从自然死亡的红棕象甲卵和幼虫尸中分离得到一株产红色色素的昆虫病原菌HN-1菌株,经形态学鉴定、生理生化测定和分子鉴定,确定为:为粘质沙雷氏菌亚种(Serratia marcescens subsp)；16S rDNA通用引物扩增该菌得到了预期的1 408 bp条带,并与粘质沙雷氏菌亚种Serratia marcesce...
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| 关 键 词： | 粘质沙雷氏菌 红棕象甲 16S rDNA序列 鉴定 |
Islation and Identification of a Pathogenic Strain of Rhynchophorus ferrugineus Oliver |
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| ZHANG Jing,QIN Weiquan,YAN Wei and PENG Zhengqiang.Islation and Identification of a Pathogenic Strain of Rhynchophorus ferrugineus Oliver[J].Chinese Journal of Tropical Crops,2011,32(12):2331-2335. |
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| Authors: | ZHANG Jing QIN Weiquan YAN Wei and PENG Zhengqiang |
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| Institution: | 1 Hainan University, Environment and Plant Protection Institute
2 Coconut Research Institute, CATAS;Coconut Research Institute, CATAS;Coconut Research Institute, CATAS;Environment and Plant Protection Institute |
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| Abstract: | A pathogen HN-1 was isolated from naturally dead insect of Rhynchophorus ferrugineus Oliver's larvae and eggs. It produced scarlet pigment and was identified with morphological, physiological, biochemical test and molecular systematic analysis. HN-1 was identified as Serratia marcescens subsp. The expected 1 408 bp band was obtained by 16S rDNA universal primer amplification. The result of sequence analysis showed that the pathogenic pathogen and Serratia marcescens subsp were a subclade with a bootstrap value of 100%. The preliminary bioassay showed that the mortality would reach to 60% for larvae after infection and the hatchability would lower 80% which proved this strain exhibited potent infectivity against red palm weevil's larvae sand eggs. |
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| Keywords: | Serratia marcescens Red palm weevil 16S rDNA sequence Identification |
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