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稻瘟病菌NAD(H)激酶的生物信息学及表达分析
引用本文:陈晓峰,王琴秋,连璧,王爱荣,鲁国东.稻瘟病菌NAD(H)激酶的生物信息学及表达分析[J].热带作物学报,2011,32(11):2063-2068.
作者姓名:陈晓峰  王琴秋  连璧  王爱荣  鲁国东
作者单位:福建农林大学生命科学学院;福建农林大学生命科学学院;福建农林大学生命科学学院;福建农林大学植物保护学院;福建农林大学植物保护学院
基金项目:国家自然科学基金项目(No. 30871616);教育部科学技术研究重点项目(No. 209065);福建省教育厅重点项目(No. JA08056)。
摘    要:对稻瘟病菌(Magnaporthe oryzae)全基因组中可能的NAD(H)激酶进行生物信息学和表达分析。结果表明,该菌含有3个可能的NAD(H)激酶,均具有保守的ATP-NAD激酶结构域;系统进化分析显示,NAD(H)激酶随着物种的进化而进化,但重要的功能结构域相对保守;MoPOS5在稻瘟病菌各生长发育阶段和致病过程中都有稳定转录,表明其在维持正常的生长发育及定殖扩展过程中扮演着十分重要的角色。

关 键 词:稻瘟病菌  NAD(H)激酶  生物信息学  表达分析

Bioinformatics and Expression Analysis of NAD(H)Kinases in Magnaporthe oryzae
CHEN Xiaofeng,WANG Qinqiu,LIAN Bi,WANG Airong and LU Guodong.Bioinformatics and Expression Analysis of NAD(H)Kinases in Magnaporthe oryzae[J].Chinese Journal of Tropical Crops,2011,32(11):2063-2068.
Authors:CHEN Xiaofeng  WANG Qinqiu  LIAN Bi  WANG Airong and LU Guodong
Institution:School of Life Science, Fujian Agriculture and Forestry University;School of Life Science, Fujian Agriculture and Forestry University;School of Life Science, Fujian Agriculture and Forestry University;College of Plant Protection, Fujian Agriculture and Forestry University;College of Plant Protection, Fujian Agriculture and Forestry University
Abstract:Accumulating evidences have suggested that NAD(H) kinases could be involved in various biological processes of great concern. In this study, candidate NAD(H) kinases in M. oryzae were analyzed through bioinformatics method. The results showed that all 3 candidate NAD(H) kinases contained conserved ATP-NAD kinase domain. Phylogenomic analysis results suggested that NAD(H) kinases evolved along with the species evolution, while some key domains were still conserved. Expression analysis of one NAD(H) kinase MoPOS5 indicated that it might play a vital role in growth, development, cell differentiation and pathogenisity. All the results will facilitate to understand the biological functions of NAD(H) kinases.
Keywords:Magnaporthe oryzae  NAD(H)kinases  Bioinformatics  Expression analysis
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