| 卡那霉素叶片涂抹法田间筛选转基因苜蓿的研究 |
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| 引用本文： | 燕丽萍,夏 阳,梁慧敏,王友平,刘翠兰,王振猛,李 丽.卡那霉素叶片涂抹法田间筛选转基因苜蓿的研究[J].中国农学通报,2009,25(14):22-26. |
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| 作者姓名： | 燕丽萍 夏 阳 梁慧敏 王友平 刘翠兰 王振猛 李 丽 |
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| 作者单位： | 1. 山东省林业科学研究院,山东济南,250014
2. 山东省林业科学研究院,山东济南,250014;山东农业大学,山东泰安,271018 |
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| 基金项目： | 山东省农业良种工程项目"抗旱耐盐苜蓿新品种(系)选育及产业化开发"，山东省科技攻关项目"林木抗逆性基因工程遗传改良研究" |
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| 摘 要： | 以田间生长的苜蓿为材料进行Kan涂抹叶片筛选转基因植株最佳浓度和适宜观察时间的研究，并对筛选的抗性植株进行了PCR检测验证。结果表明，Kan叶片涂抹田间筛选的最佳浓度为1000mg/L，观察时间以12d为宜；经PCR检测验证，Kan筛选抗性苗的阳性检测率达到93.8%。研究建立了一种在田间对转基因苜蓿进行大量筛选的简便方法，对于转基因苜蓿材料的选育具有重要利用价值。
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| 关 键 词： | 生长素 生长素 赤霉素 油菜素内酯 乙烯 相互作用 |
| 收稿时间： | 2009-02-05 |
| 修稿时间： | 2009-02-08 |
Study on the Field Screening for Transgenic Alfalfa by Smearing Kanamycin on the Leaves |
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| Yan Liping,Xia Yang,Liang Huimin,WangYouping,Liu Cuilan,Wang Zhenmeng,Li Li.Study on the Field Screening for Transgenic Alfalfa by Smearing Kanamycin on the Leaves[J].Chinese Agricultural Science Bulletin,2009,25(14):22-26. |
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| Authors: | Yan Liping Xia Yang Liang Huimin WangYouping Liu Cuilan Wang Zhenmeng Li Li |
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| Abstract: | Kanamycin can be used to select the transformed plants containing the nptⅡgene. In order to assay the optimal screening concentration to nptⅡ gene, Kanamycin was smeared to the surface of the transformed alfalfa leaves. It showed that 1000mg/L Kanamycin with 12 days’ selecting was best. This method was compared with PCR as well, and the results suggested that the equivalent ratio was 93.8 % in the resistant plants. Accordingly, a simple method of filed screening gene transgenic alfalfa has been made. This result is very useful for the transgenic alfalfa to be screened and cultivated. |
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| Keywords: | Kan |
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