| 狂犬病病毒糖蛋白、核蛋白融合基因原核质粒的构建与表达 |
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| 引用本文： | 姜海龙,钱爱东. 狂犬病病毒糖蛋白、核蛋白融合基因原核质粒的构建与表达[J]. 吉林农业大学学报, 2006, 28(3): 313-316 |
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| 作者姓名： | 姜海龙 钱爱东 |
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| 作者单位： | 吉林农业大学动物科技学院,长春,130118;吉林农业大学动物科技学院,长春,130118 |
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| 摘 要： | 试验将编码狂犬病病毒糖蛋白、核蛋白融合基因克隆到原核表达载体pET-32a( )中,构建狂犬病病毒原核表达质粒。将该质粒转化到大肠杆菌BL21中,经IPTG诱导表达出约2.09×104D的狂犬病病毒糖/核融合基因编码的蛋白质。Western-blotting检测结果表明,该融合蛋白质具有免疫原性。
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| 关 键 词： | 狂犬病病毒 糖蛋白 核蛋白 原核表达质粒 共表达 |
| 文章编号： | 1000-5684（2006）03-0313-04 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-06-202005-10-15 |
Construction of Prokaryotic Expression Plasmid with G and N Genes of Rabies Virus and Co-Expression of the Two Genes |
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| JIANG Hai-long,QIAN Ai-dong. Construction of Prokaryotic Expression Plasmid with G and N Genes of Rabies Virus and Co-Expression of the Two Genes[J]. Journal of Jilin Agricultural University, 2006, 28(3): 313-316 |
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| Authors: | JIANG Hai-long QIAN Ai-dong |
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| Abstract: | The experiment was conducted to construct prokaryotic expression plasmid containing the G and N melt genes of rabies virus through cloning the genes of rabies virus into pET-32a( ).After transforming the plasmid into E. coli BL21,the bacteria were induced by IPTG and the protein was detected with western-blotting test.The result indicated that the two genes could be co-expressed by the prokaryotic expression plasmid,which will supply foundation for the preparation of the antigen use to diagnose rabies. |
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| Keywords: | rabies virus glycoprotein neucleoprotein prokaryotic expression plasmid co-expression |
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