| 白桦抗虫基因转化的初步研究 |
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| 引用本文： | 詹亚光,刘志华,王玉成,王志英,杨传平,刘桂丰. 白桦抗虫基因转化的初步研究[J]. 东北林业大学学报, 2001, 29(6): 4-6 |
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| 作者姓名： | 詹亚光 刘志华 王玉成 王志英 杨传平 刘桂丰 |
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| 作者单位： | 东北林业大学, |
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| 基金项目： | 东北林业大学校基金课题资助 |
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| 摘 要： | 用根癌农杆菌介导法对白桦（Betula platyphylla)进行转化，获得了转化再生植株。卡那霉素抗性愈伤组织产生率达10%-22%，抗性不定枝的生根率达80%以上。经分子生物学检测，在卡那霉素抗性转化植株中，有34%检测出GUS活性，76.5%的卡那霉素抗性植株的Southern斑点杂交呈阳性反应。初步证明，抗虫基因已转入白桦中。
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| 关 键 词： | 白桦 抗虫基因 基因转化 蜘蛛杀虫肽基因 农杆菌介导 卡那霉素 |
| 修稿时间： | 2001-07-23 |
Transformation of Insect Resistant Gene into Birch/ |
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| Zhan Yaguang,Liu Zhihua,Wang Yucheng,Wang Zhiying,Yang Chuanping,Liu Guifeng. Transformation of Insect Resistant Gene into Birch/[J]. Journal of Northeast Forestry University, 2001, 29(6): 4-6 |
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| Authors: | Zhan Yaguang Liu Zhihua Wang Yucheng Wang Zhiying Yang Chuanping Liu Guifeng |
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| Abstract: | Transgenic plants were obtained by Agrobacterium-mediated transfer system from birch leaf, stem segment and leaf stalk. Gus detection shows that 34% of kanamycin resistant plants have Gus activity. DNA dot analysis indicates that 76.5% give positive reaction. These results elementaly proved that insect resistant gene(spider insecticidal peptide gene) is transferred and integrated into birch genome. |
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| Keywords: | Birch Transformation Insect resistance |
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