| 用非放射性原位杂交技术定位家猪PGD和HSL基因 |
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| 引用本文： | 蔡更元,熊远著.用非放射性原位杂交技术定位家猪PGD和HSL基因[J].华中农业大学学报,1998,17(4):369-372. |
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| 作者姓名： | 蔡更元 熊远著 |
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| 作者单位： | 华中农业大学猪遗传改良农业部重点实验室 |
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| 摘 要： | 用地高辛为标记物，以随机引物法标记猪ＰＧＤ ｃＤＮＡ和ＨＳＬ ｃＤＮＡ探针，经与半微量全血培养法制备的染色体进行原位杂交后，将猪ＰＧＤ和ＨＳＬ基因分别定位在猪６号染色体６ｑ＾２５和６ｃｅｎ－６ｑ＾２１区域。
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| 关 键 词： | 猪 原位杂交 PGD基因 HSL基因 基因定位 |
ASSIGNMENET OF THE PGD AND HSL GENES IN PIGS BY USING NONRADIOACTIVE in situ HYBRIDIZATION |
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| Cai Gengyuan,Xiong Yuanzhu,Deng Changyan,Jiang Siwen,Wu Zhengfang.ASSIGNMENET OF THE PGD AND HSL GENES IN PIGS BY USING NONRADIOACTIVE in situ HYBRIDIZATION[J].Journal of Huazhong Agricultural University,1998,17(4):369-372. |
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| Authors: | Cai Gengyuan Xiong Yuanzhu Deng Changyan Jiang Siwen Wu Zhengfang |
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| Abstract: | Porcine partial PGD cDNA and HSL cDNA were labeled with degoxigenin with random primed labeling method, then used in chromosomal in situ hybridization. The porcine PGD and HSL gene were mapped to 6q 25 and 6cen-q 21 , respectively. |
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| Keywords: | domestic pig nonradioactive in situ hybridization phosphogluconate dehydrogenase(PGD) gene hormone sensitive lipase(HSL) gene |
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