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Suppression of hepatitis B virus replication in Tupaia hepatocytes by tumor necrosis factor alpha of Tupaia belangeri |
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| Authors: | Xu Yang Köck Josef Lu Yinping Yang Dongliang Lu Mengji Zhao Xiping |
| Institution: | a Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China b Institute of Virology, University Hospital of Essen, Essen 45122, Germany c Department of Internal Medicine II, University Hospital of Freiburg, Freiburg 79106, Germany d Division of Clinical Virology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China e Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China |
| Abstract: | Recently, Tupaia belangeri was used to study the full replication cycle of hepatitis B virus (HBV) in the primary hepatocyte cultures. Thus, the Tupaia model represents a suitable model to study the effects of cytokines on HBV infection. Here, Tupaia tumor necrosis factor-alpha (TNF-α) was molecularly cloned and expressed in mammalian cells. A test system for the biological activity of Tupaia TNF-α was established on the basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant Tupaia TNF-α was able to suppress HBV replication in primary Tupaia hepatocytes (PTH). However, the formation of HBV covalently closed circular DNA (cccDNA) and viral RNA was not completely prevented. Therefore, Tupaia TNF-α may contribute significantly to the control of HBV infection though it is not able to completely inhibit HBV replication alone. The characterization of this important cytokine allows further studies on its antiviral actions in the Tupaia model. |
| Keywords: | Tumor necrosis factor-alpha Tupaia Hepatitis B virus |
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