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Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India |
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| Authors: | Kaur P Filia G Singh S V Patil P K Ravi Kumar G V P P S Sandhu K S |
| Affiliation: | a Department of Epidemiology and Preventive Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India b Veterinary Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, P.O. Farah, Mathura 281122, UP, India c Department of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India |
| Abstract: | Johne's disease is chronic granulomatous infectious enteritis of animals caused by Mycobacterium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from different districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected to Ziehl-Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl-Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’ type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’ type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of ‘Cattle’ type Map from buffalo and goat species in India. |
| Keywords: | Johne's disease Mycobacterium avium subspecies paratuberculosis Ziehl-Neelsen staining PCR IS900 IS1311 REA Molecular epidemiology |
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