| 海南省木豆丛枝病植原体的分子检测及鉴定 |
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| 引用本文： | 车海彦,罗大全,符瑞益,吴云锋. 海南省木豆丛枝病植原体的分子检测及鉴定[J]. 植物病理学报, 2010, 40(2): 113-121 |
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| 作者姓名： | 车海彦 罗大全 符瑞益 吴云锋 |
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| 作者单位： | 1 西北农林科技大学植物保护学院与陕西省农业分子生物学重点实验室, 植保资源与病虫害治理教育部重点实验室, 杨凌 712100;2 中国热带农业科学院环境与植物保护研究所, 儋州 571737 |
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| 基金项目： | 国家自然科学基金，高等学校学科创新引智计划 |
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| 摘 要： | 利用植原体通用引物R16mF2/R16mR1和rp (Ⅱ) F1/rp (Ⅱ) R1对海南木豆丛枝病植原体16S rDNA和部分核糖体蛋白(ribosomal protein,rp)基因序列进行PCR扩增、克隆和测序。获得海南木豆丛枝病植原体16S rDNA基因片段为1430bp,rp基因片段为1170bp。核苷酸同源性比较和系统进化树构建表明,引起海南木豆丛枝病的植原体应属于16SrⅡ组中的亚组ⅲ。本研究首次从分子水平确定了引起我国海南木豆丛枝病的病原物为植原体,明确了其分类地位,为该病害流行学研究和防治提供了理论依据。
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| 关 键 词： | 海南木豆丛枝病 植原体 16S rDNA rp基因 序列分析 |
| 收稿时间： | 2009-04-16 |
Molecular detection and identification of a phytoplasma associated with pigeon pea witches'-broom disease in Hainan Province,China |
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| CHE Hai-yan,LUO Da-quan,FU Rui-yi,Wu Yun-feng. Molecular detection and identification of a phytoplasma associated with pigeon pea witches'-broom disease in Hainan Province,China[J]. Acta Phytopathologica Sinica, 2010, 40(2): 113-121 |
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| Authors: | CHE Hai-yan LUO Da-quan FU Rui-yi Wu Yun-feng |
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| Affiliation: | 1 College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A & F University, Yangling 712100, China;2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
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| Abstract: | Primers R16mF2/R16mR1 and rp (Ⅱ)F1/rp (Ⅱ)R1 were used to amplify phytoplasma DNA from infected pigeon pea samples, then the amplified fragments were cloned and sequenced. The 16S rDNA (1 430 bp) and rp gene (1 170 bp) were amplified from infected pigeon pea. Homology analysis and phylogenetic tree showed that Hainan pigeon pea witches'-broom phytoplasma belonged to the 16S rⅡ group, ⅲ subgroup. Pigeon pea witches'-broom phytoplasma was firstly identified by molecular biotechnology and confirmed its taxonomic status in Hainan, it provided a theoretical basis for research on the epidemiology and control of the disease. |
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| Keywords: | 16S rDNA |
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