基于Linux＋Apache＋MySQL＋PHP的棉花分子生物学数据库系统构建

 利用课题组积累的数据信息和网上公用数据库中有关棉花分子生物学数据，采用MySQL为后台数据库，以Apache＋PHP为环境，在Linux操作系统下实现了棉花分子生物学数据库系统的构建和wwwBLAST的本地化服务。在脱机状态下使用blast进行同源性比对，使得数据查询和序列比对更加方便、快捷、稳定和安全，而且增强了针对性。数据库最终以局域网的形式发布，通过授予权限可向同行提供利用。本课题组在棉纤维品质、黄萎病抗性等相关基因的克隆研究中使该数据库得到初步应用。

The Construction of Cotton Molecular Biology Database System Based on Linux+Apache+MySQL+PHP

With the rapid development of biotechnology and the exponent increase of cotton molecular biology data, in order to further dispose, analyze and use these data, it is necessary to establish the corresponding bioinformatics databases. Based on the research data of our laboratory and of public cotton molecular biology, we constructed a cotton molecular biology database system in the Linux operating system. In the database system, MySQL database was used as a background, and the open environment depended on Apache+PHP. In the Linux operating system, the standalone of wwwBLAST alignment system services was accomplished based on Apache environment. The blast could be operated in the off line state. The service could make up the blast deficiencies of public databases which were complicated and of weak pertinence. Since the local data were added into databases, the analysis and comparison of data became safer, quicker and more detailed. The database was put out by local area network and could be accessed at author’s permission. This database, in our laboratory, has been put into application in related gene cloning of fiber development and Verticillium wilt resistance.