| 猪THRSP基因5'调控区多位点SNP联合效率的研究 |
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| 引用本文： | 谢亚男,焦明慧,陈宏权,潘中婷,陈公伟,周梅,王学故,马帮军.猪THRSP基因5'调控区多位点SNP联合效率的研究[J].安徽农业大学学报,2014,41(2):278-281. |
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| 作者姓名： | 谢亚男 焦明慧 陈宏权 潘中婷 陈公伟 周梅 王学故 马帮军 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥230036;安徽农业大学动物科技学院,合肥230036;安徽畜禽遗传资源保护与生物育种省级实验室,合肥230036 |
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| 基金项目： | 国家自然科学基金（31172180）和安徽省科技攻关项目（12010302052）共同资助。 |
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| 摘 要： | 旨在分析猪THRSP基因5’调控区多位点SNP联合调控THRSP基因表达的效率。实验从猪基因组获取包含多位点SNP的目的片段，利用荧光素酶报告基因pGL3-Basic构建表达载体，检测多位点SNP 的联合启动效率，并通过实时荧光定量PCR进行THRSP基因表达验证分析。结果表明，在5个SNP位点中，TCAGA 的启动效率最高，比CTGGA的启动效率高129％，而CCAGA、CTAGA和CTGAT的启动效率较CTGGA分别低99.0%、73.2％和42.6％。TCAGA的启动效率较CCAGA高75.6％，而CCAGA比CTAGA的启动效率高96.6％；报告基因所揭示的SNP启动效率与THRSP基因实时荧光定量PCR结果一致。
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| 关 键 词： | THRSP基因 多位点SNP联合调控 基因表达 猪 脂肪沉积 |
Study on the promoter efficiency of multi-sites SNP in 5' regulatory region of THRSP gene in pigs |
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| XIE Yanan,JIAO Minghui,CHEN Hongquan,PAN Zhongting,CHEN Gongwei,ZHOU Mei,WANG Xuegu and MA Bangjun.Study on the promoter efficiency of multi-sites SNP in 5' regulatory region of THRSP gene in pigs[J].Journal of Anhui Agricultural University,2014,41(2):278-281. |
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| Authors: | XIE Yanan JIAO Minghui CHEN Hongquan PAN Zhongting CHEN Gongwei ZHOU Mei WANG Xuegu and MA Bangjun |
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| Institution: | School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Breeding, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | |
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| Keywords: | THRSP gene multi-sites SNP regulation gene expression pigs fat deposition |
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