酶抑制率法在4种蔬菜农药残留快速检测中的应用

目的]探究蔬菜农药残留的快速测定方法的准确性。方法]使用酶抑制率法对胜利农场的本地温室大棚、露地常用蔬菜及南方韭菜等蔬菜的毒死蜱、敌百虫、氧化乐果和呋喃丹(克百威)4种有机磷和氨基甲酸酯类农药进行了残留检测。并用该法在实验室内对这4种农药灵敏度进行了检测。同时,对易产生假阳性的蔬菜如韭菜、生姜进行加热处理,并把抑制率35%以上的蔬菜用色谱法检测验证。结果]本地夏季的小白菜和冬季超市的南方韭菜农药残留超标,韭菜中毒死蜱残留超标近6倍。抑制率20%时,氧化乐果缓冲溶液的浓度最低只能达到3 mg/kg,而敌百虫、呋喃丹、毒死蜱缓冲液的浓度分别可达0.001、0.001、0.030 mg/kg。结论]酶抑制率法对氧化乐果检测灵敏度低,对敌百虫、呋喃丹、毒死蜱灵敏度高。

Application of Enzyme Inhibition Rate Method in Rapid Detection of Four Vegetable Pesticide Residues

Objective ] To explore the accuracy of rapid detection of vegetable pesticide residues. Method] Enzyme inhibition rate method was used to detect trichlorfon, carbofuran, chlorpyrifos, ometboate residue in greenhouse and common vegetables in Shengli Farm and south Chinese chive. Heating treatment was carried out on Chinese chive and ginger, inhibition rate above 35% vegetables were verified with chromatography method. Result] The results showed that the pesticide residue of local summer Chinese cabbage and south Chinese chive in winter in supermar- ket exceed the standard, ehlorpyrifos residue in Chinese chive exceeds nearly 6 times. When inhibition rate is 20%, the minimum concentration of omethoate buffer solution is 3 mg/kg, while concentration of trichlorfon, carbofuran, chlorpyrifos buffer solution are0. 001 mg/kg, 0. 001 mg/kg, 0. 030 mg/kg. Conclusion] Enzyme inhibition rate method has low sensitivity for detecting omethoate, high sensitivity for trichlorfon, carbofuran, chlorpyrifos.