冀东地区野生大豆愈伤组织诱导及其耐盐性

以冀东沿海及内地不同条件下生长的14个野生大豆为试验材料，选取下胚轴和子叶作为外植体，在四种不同成分的培养基上诱导愈伤组织，经继代观察愈伤组织的质量，同时，在含不同浓度NaCl(0,0.2%,0.4%,0.6%,0.8%,1.0%)的继代培养基上，观察愈伤组织的耐盐性，试验结果表明；诱导野生大豆愈伤组织的适宜培养基为MS 2，4-D2.0mg/L 6-BA0.5mg/L 蔗糖5% 琼脂0.8%；不同材料之间愈伤组织的耐盐性有明显差异；耐盐性强的可在0.8%NaCl浓度下生存低的只能忍耐0.2%NaCl含量；本试验确定野生大豆愈伤组织耐盐性鉴定适宜的浓度为0.6%左右；盐碱条件下生长的野生大豆一般耐盐性较强，非盐碱环境下生长的野生大豆耐盐性也存在着耐盐性较强的类型，说明生长环境与细胞水平的耐盐性之间并没有完全的对应关系。

THE STUDY ON SALT-TOLERANCE ABILITY AND INDUCTION OF CALLUS OF WILD SOYBEAN IN EASTERN OF HEBEI PROVINCE

The 14 genotypes of wild soybean growing at different conditions in eastern of Hebei province were studied in this paper. Hypocotyls and cotyledon were used as explants, through tissue culture, calli was induced on 4 kinds of medium containing different composition. The calli were subcultured on D 4 medium for 4 times. The salt-tolerance of calli was observed on different medium containing 0.0,0.2,0.4,0.6,0.8,1.0%NaCl. Ability of salt-tolerance was identified through growth amount,quality,becoming brown and death ratio of calli. The results showed that MS+2,4-D2.0mg/L+6-BA0.5mg/L+sucrose 5%+agar 0.8% is the best medium for callus induction. Salt-tolerance ability of calli had obvious different among different wild soybean. The stronger salt-tolerance wild soybean could survive at the concentration of 0.8% NaCl. The ability of salt-tolerance of 6?7?10?11?12?14 was stronger, the growth amount and quality of calli descended with the rising of concentration of NaCl. The experiment indicated that screening concentration for salt-tolerance of wild soybean is 0.6%NaCl. Generally, the wild soybeans from saline soil have stronger ability of salt-tolerance ,where as, the wild soybean from non-saline environment have very strong existing ability in NaCl medium, too. There was not complete correspondence relation between growth environment and salt-tolerance ability at cell level The results suggested that the relation of salt-tolerance ability at cell level and plant level should study further.weengrowthenviron