| 高效液相色谱法测定不同品种辣椒中的辣椒素 |
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| 引用本文： | ZHANG Hai-li,李焕秀,LIU Wei-guo,孙娟,YANG Yong-chao,陈佳.高效液相色谱法测定不同品种辣椒中的辣椒素[J].四川农业大学学报,2008,26(2). |
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| 作者姓名： | ZHANG Hai-li 李焕秀 LIU Wei-guo 孙娟 YANG Yong-chao 陈佳 |
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| 作者单位： | 四川农业大学林学园艺学院,四川,雅安,625014 |
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| 摘 要： | 研究了用高效液相色谱法测定辣椒中的辣椒素。色谱柱为Hypersil ODS柱(4.0mm×250mm,5μm)UV检测器波长为280nm。流动相为V(CH3OH)∶V(H2O)=75∶25,流速1.2mL/min。结果表明:线性回归方程是y=1042106.11x+2.7929717相关系数为R=0.99999样品的精密度RSD=2.6%,平均回收率为97.39%,并对12个辣椒品种中的辣椒素进行测定,辣椒素含量最高的是野山椒-1,最低的是樱桃椒。
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| 关 键 词： | 高效液相色谱 辣椒素 辣椒 |
Determination of Capsicin in Different Variety of Capsicum by High Performance Liquid Chromatography |
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| ZHANG Hai-li,LI Huan-xiu,LIU Wei-guo,SUN Juan,YANG Yong-chao,CHEN Jia.Determination of Capsicin in Different Variety of Capsicum by High Performance Liquid Chromatography[J].Journal of Sichuan Agricultural University,2008,26(2). |
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| Authors: | ZHANG Hai-li LI Huan-xiu LIU Wei-guo SUN Juan YANG Yong-chao CHEN Jia |
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| Institution: | ZHANG Hai-li1,2,LI Huan-xiu2,LIU Wei-guo2,SUN Juan2,YANG Yong-chao2,CHEN Jia2 |
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| Abstract: | The determination of capsicin in pepper was studied by means of high performance liquid chromatography(HALP).The HPLC system consisted of Hypersil ODS(4.0 mm×250 mm,5 μm)and UV detector set at 280 nm.The mobile phase was V(CH3OH)∶V(H2O)= 75∶25 with a flow rate of 1.2 mL/min.The results showed that the linear equation was y=1042106.11x+2.79 and correlation coefficient was 0.99999.The average recovery was 97.39%.Capsicin in 12 different varieties of capsicum were determined,the one that contains maximum capsicin is wild pepper-1,minimum one is cherry pepper. |
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| Keywords: | high performance liquid chromatography capsicin capsicum |
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