叶用莴苣组织培养再生体系的建立

为了满足市场需求，通过组织培养技术可以使叶用莴苣不受季节气候所牵制。随时可以工厂化大量生产，以达到百姓日食之需。本研究以PS11叶用莴苣为试材，从种子开始培育无菌苗，MS培养基为基础培养基，附加不同浓度的6-BA和NAA激素配比，再经愈伤组织诱导、芽分化、生根3个步骤的离体培养，建立叶用莴苣快速高效的再生体系。研究表明：该品种最佳诱导愈伤组织分化的培养基为MS+0.2mg/L6-BA+0.4mg/LNAA，出愈率达到100%，出芽率达到71.6%。

Establishment of Tissue Culture Regeneration System of leaf Lettuce

Abstract: In order to meet market demand, Through the Tissue Culture Technology can make the leaf lettuce is not subject to seasonal limate.Always ready to industrialized mass production , in order to reach the people of the solar eclipse. In this study taking PS11 of leaf lettuce as test material, starting from the seed to cultivate aseptic seedling on MS basic culture medium with different consistency of NAA or 6-BA. supple mented with different concentrations of 6-BA and NAA.Through the callus induction, bud differentiation and rooting in 3 steps,leaf lettuce fast and efficient regeneration system is established.Research shows that: the best medium varieties induced callus differentiation of MS 0.2 mg/L 6-BA 0.4 mg/L NAA, the callus induction rate reached 100%, the budding rate reached 78.3%.