内源性绵羊肺腺瘤病毒NM株pol基因的克隆与序列分析

参照GenBank中内源性绵羊肺腺瘤病毒enJS56A1株pol基因序列设计了2对引物。应用PCR技术特异性地扩增出病毒的pol基因片段,将其克隆到PMD19-T载体后测序。应用计算机软件将测定序列与内源性南非代表毒株enJS56A1(AF153615)的pol基因序列比较,核苷酸同源性为99.3%,推导出的氨基酸同源性为95.0%。与外源性南非代表株JSRV-SA(M80216)的pol基因序列比较,核苷酸同源性为99.0%,氨基酸同源性为99.3%。这也是我国首次报道的内源性绵羊肺腺瘤病毒pol基因序列,为我国科研工作者进行更深入的研究奠定基础。

Cloning and sequence analysis of pol gene of the Inner Mongolia strain of endogenous jaagsiekte sheep retrovirus

According to the published pol gene sequence of enJS56A1 strain in GenBank,two pairs of primers were designed.A pol gene was amplified by PCR and then was cloned into PMD19-T vector and then sequenced.The acquired nucleotide sequences were analysed by computer softwares.As a result,The nucleotide and amino acid sequences of NM strain pol gene were compared with the counterpart sequences of South Africa enJS56A1 strain(AF153615) and South Africa JSRV-SA strain(M80216).The nucleotide and amino acid homology of pol gene were 99.3%,95.0% and 98.8%,98.8%,respectively.This is the first nucleotide sequence of enJSRV reported in China.These results provide information for future developments on OPA.