铜(Ⅱ)-多酚纳米复合物对水培营养液中藻类生长的抑制

为研究铜(Ⅱ)-多酚纳米复合物对水培营养液中藻类生长的抑制作用及对生菜的影响，将金属离子(Cu²⁺)和天然植物多酚(杨梅单宁BT、橡椀单宁VT、塔拉单宁Tr、单宁酸TA)自组装形成铜(Ⅱ)-多酚纳米复合物，设置铜(Ⅱ)-多酚纳米复合物抑制小球藻试验和实际水培生菜试验。结果表明：1)多酚诱导的界面相互作用促使铜(Ⅱ)-多酚纳米复合物能够黏附于藻类细胞表面并缓慢释放铜离子，从而引起了藻细胞的氧化损伤和藻细胞的死亡，可实现长期有效的抑藻效果。其中，铜与杨梅单宁BT形成的Cu^Ⅱ-BT使用7 d后，对小球藻的抑制率仍然可达到99%。2) Cu²⁺与缩合类植物多酚(杨梅单宁BT、橡椀单宁VT)自组装形成的Cu^Ⅱ-BT和Cu^Ⅱ-VT对藻类生长的抑制能力优于Cu²⁺与水解类植物多酚(塔拉单宁Tr、单宁酸TA)自组装形成的Cu^Ⅱ-Tr和Cu^Ⅱ-TA。3)铜(Ⅱ)-多酚纳米复合物能够在实际生产中抑制营养液中藻类的生长，并且与对照相比...

Inhibition effects of copper(II)-phenolic nanocomplexes on the growth of algae in hydroponic nutrient solution

The inhibition effects of copper(II)-phenolic nanocomplexes on algal growth and its effect on lettuce in hydroponics were studied. In this study, natural polyphenols(Bayberry tannin, Valonia tannin, Tara tannin, and Tannin acid)and functional metal ions(copper ions)were used as building blocks to engineer self-assembled copper(II)-phenolic nanocomplexes, and the inhibition experiment of copper(II)-phenolic nanocomplexes for Chlorella vulgaris and application of copper(II)-phenolic nanocomplexes on actual hydroponic lettuce were designed. The results showed that: 1)The polyphenol-induced interface interaction enabled the attachment of copper(II)-phenolic nanocomplexes on the surface of algal cells, which continuously and precisely delivered metal ions(copper ions)to algae, resulting in oxidative damage and cell death. Thus, the copper(II)-phenolic nanocomplexes presented long-lasting inhibition for algae, and the corresponding inhibition rate of Cu^II-BT still reached 99% on the 7th day for C. vulgaris. 2)The algal inhibition experiments showed that the inhibition ability of Cu^II-BT and Cu^II-VT for C. vulgaris was better than that of Cu^II-Tr and Cu^II-TA. 3)Plant experiments showed that copper(II)-phenolic nanocomplexes could inhibit algae during actual production, and there was no difference in fresh weight of lettuce seedlings compared with the control group. These results demonstrate the copper(II)-phenolic nanocomplexes had greater biosafety. Thus, the self-assembled copper(II)-phenolic nanocomplexes could inhibit algal blooms in actual hydroponic production, which contributed to reduce the problem of loss of crop yield caused by algal blooms.