Pseudomonas syringae pv. maculicola in Australia: pathogenic, phenotypic and genetic diversity |
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| Authors: | B. J. Peters &dagger ,G. J. Ash,E. J. Cother,D. L. Hailstones,D. H. Noble, N. A. R. Urwin |
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| Affiliation: | Farrer Centre, School of Agriculture, Charles Sturt University, PO Box 588, Wagga Wagga, 2678 New South Wales;;Orange Agricultural Institute, Forest Road, Orange, 2800 New South Wales;and;Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, 2570 New South Wales, Australia |
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| Abstract: | Pseudomonas syringae pv. maculicola causes bacterial leaf spot on cruciferous plants in Australia. This is the first record confirming the identity of seven isolates currently stored as P. syringae pv. maculicola in the herbarium of the Department of Agriculture, Orange, Australia (Herb. DAR). The isolates were identified using pathogenicity testing on cauliflower and fatty acid methyl ester analysis. They clustered together using repetitive sequence-based polymerase chain reaction (rep-PCR) and PCR-restriction fragment-length polymorphism (RFLP) of the 16S−23S internal transcribed spacer region (ITS) using seven restriction enzymes. Identification was confirmed by comparison of these isolates with known overseas isolates of P. syringae pv. maculicola , but there was significant variation in their pathogenicity and genetic structure. |
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| Keywords: | crucifers fatty acids pathogenicity RFLP rep-PCR |
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