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| 大叶金花草多糖的提取、分离纯化及结构分析 | |
| 引用本文： | 罗娅君,肖新峰,王照丽.大叶金花草多糖的提取、分离纯化及结构分析[J].林产化学与工业,2009,29(1):68-72. |
| 作者姓名： | 罗娅君 肖新峰 王照丽 |
| 作者单位： | 1. 绵阳师范学院,化学与化学工程系,四川,绵阳,621000 2. 山东科技大学,化学与环境工程学院,山东,青岛,261000 3. 成都市环境保护研究院,四川,成都,610065 |
| 基金项目： | 四川省教育厅青年基金，绵阳师范学院科研基金 |
| 摘 要： | 采用热水提取法从大叶金花草中提取大叶金花草多糖,用酶法和Seveg法去除蛋白质,H2 O2脱色,经DEAE-纤维素(Cl -)柱层析纯化后,纸层析和Sephadex G-100 凝胶柱层析证实为单一组分,并用凝胶色谱法、薄层层析、乙酰化GC-MS、紫外光谱和红外光谱等对其组成和结构进行了研究.大叶金花草多糖平均分子质量为8.6×104 u,由阿拉伯糖、葡萄糖和糖醛酸组成,在260和 280 nm处有多糖无核酸和蛋白质的吸收峰.大叶金花草多糖是含有β-吡喃葡萄糖的非蛋白类单一多糖. |
| 关 键 词： | 大叶金花草 多糖 分离纯化 结构分析 |
Extraction,Separation,Purification and Structural Analysis of Polysaccharide from Stenoloma chusanum(Linn.)Ching |
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| LUO Ya-jun,XIAO Xin-feng,WANG Zhao-li.Extraction,Separation,Purification and Structural Analysis of Polysaccharide from Stenoloma chusanum(Linn.)Ching[J].Chemistry & Industry of Forest Products,2009,29(1):68-72. | |
| Authors: | LUO Ya-jun XIAO Xin-feng WANG Zhao-li |
| Institution: | 1.Department of Chemistry and Chemical Engineering;Mianyang Normal University;Mianyang 621000;China;2.College of Chemical and Environmental Engineering;Shandong University of Science & Technology;Qingdao 261000;3.Chengdu Academy of Environmental Protection;Chengdu 610065;China |
| Abstract: | Water-soluble polysaccharide in Stenoloma chusanum(Linn.)Ching is extracted by means of hot water.Polysaccharide is purified by enzyme treatment and Seveg method to remove protein,then decolorized by 10 % H2O2 to remove pigment,followed by DEAE-cellulose column chromatography.Polysaccharide is shown to be a single homogeneous component by PC and Sephadex G-100 column chromatography,and their structural composition was studied by means of permeation chromatography(GPC),TLC,GC-MS,UV,IR,etc..The average molecu... |
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