稻瘟病菌生理小种RAPD分析及其与马唐瘟的差异

氯化卞法微量提取稻瘟病菌７个生理小各和马唐瘟病菌基因组ＤＮＡ，用６０个随机引物进行ＰＣＲ扩增，其中２１个引物的特异性扩增条带，第一引物－菌系组合扩增到３－１９条，平均１０．３条；其中有多态性条速为１－１２条，平均６．１条，多态性为６１．１％。说明稻瘟病菌群体有丰富的ＲＡＰＤ多态性。引物ＯＰＨ１、ＯＰＨ２，ＯＰＨ４，ＯＰＨ１３，ＯＰＨ１４，ＯＰＫ１４等扩增的条带多，多态性丰富，而且可以有效地区分稻瘟

RAPD Analysis of Physiological Races of Rice Blast Fungus (Pyricularia grisea) and Its Difference to Digitaria Blast

The genomic DNA of 7 rice physiological races and 1 weed [Digitaria sanguinalis(L.) Scop. jisolote of Pyricularia grlsea were obtained by using benzyl chloride.The Random Amplified Polymorphic DNA (RAPD)analysis was conducted with 21 effecti ve primers of 60 random decamer primers tested. Three to nineteen bands, averaging 10. 3, were amplified for each primer -isolate combination with 1- 12 polymorphic markers, averaging 6. 1, revealing that rice blast fungus was abundant in RAPD. Numerous bands and polymorphic markers were produced by such primers as OPH 1, OPH2, OPH4,OPH 13, OPH 14 and OPK 14, and the difference between the races of rice blast was easily identified. The difference of the fungi between rice blast and Digitaria b1ast could be identified by almost all of the 21 primers, and the products amplified by some primers,such as OPG3, OPG 13 and OPK 1, showed very few bands in the rice races but abundant in the Digitaria isolate, suggesting thcir genetic diversity.