牛DYRK2 基因的电子克隆及生物信息学分析

以人DYRK2 基因cDNA 序列为探针，通过EST 数据库进行同源性筛选，对牛DYRK2 基因进行电子克隆。序列分析结果表明，牛DYRK2 基因包含一个1 581 bp 的开放阅读框架，共编码526 个氨基酸；蛋白特征分析显示，牛DYRK2 蛋白的分子式为C2632H4204N762O761S26，相对分子质量为59 532.5，理论等电点pI 为9.53。结果表明牛DYRK2 基因与羊、人等其他动物的DYRK2 具有较高的一致性。

In silico cloning and bioinformatic analysisof DYRK2 gene in cattle

Using human DYRK2 cDNA sequence as a probe, screening homology sequences in the EST database, theDYRK2 gene from cattle was cloned by silico cloning. Sequence analysis showed that cattle DYRK2 cDNA contained anopen reading frame(ORF) with 1 581 bp which encoded 526 amino acids. Protein analysis found that the molecular formulaof encoded protein DYRK2 of cattle was C2632H4204N762O761S26, with weight of 59 532.5 and theoretical pI of 9.53. The resultindicated that the nucleotide sequences of the coding region between cattle, sheep, people and other animals were quiteconservative.